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Am J Physiol Cell Physiol 277: C74-C82, 1999;
0363-6143/99 $5.00
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Vol. 277, Issue 1, C74-C82, July 1999

Cholecystokinin induction of mob-1 chemokine expression in pancreatic acinar cells requires NF-kappa B activation

Bing Han and Craig D. Logsdon

Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109

Inflammatory mediators are involved in the early phase of acute pancreatitis, but the cellular mechanisms responsible for their generation within pancreatic cells are unknown. We examined the role of nuclear factor-kappa B (NF-kappa B) in cholecystokinin octapeptide (CCK-8)-induced mob-1 chemokine expression in pancreatic acinar cells in vitro. Supraphysiological, but not physiological, concentrations of CCK-8 increased inhibitory kappa B (Ikappa B-alpha ) degradation, NF-kappa B activation, and mob-1 gene expression in isolated pancreatic acinar cells. CCK-8-induced Ikappa B-alpha degradation was maximal within 1 h. Expression of mob-1 was maximal within 2 h. Neither bombesin nor carbachol significantly increased mob-1 mRNA or induced Ikappa B-alpha degradation. Thus the concentration, time, and secretagogue dependence of mob-1 gene expression and Ikappa B-alpha degradation were similar. Inhibition of NF-kappa B with pharmacological agents or by adenovirus-mediated expression of the inhibitory protein Ikappa B-alpha also inhibited mob-1 gene expression. These data indicate that the NF-kappa B signaling pathway is required for CCK-8-mediated induction of mob-1 chemokine expression in pancreatic acinar cells. This supports the hypothesis that NF-kappa B signaling is of central importance in the initiation of acute pancreatitis.

pancreas; pancreatitis; cytokines; adenovirus


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