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Am J Physiol Cell Physiol 276: C1405-C1410, 1999;
0363-6143/99 $5.00
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Vol. 276, Issue 6, C1405-C1410, June 1999

Malignant human gliomas express an amiloride-sensitive Na+ conductance

James K. Bubien1, Deborah A. Keeton1, Catherine M. Fuller1, G. Yancey Gillespie2, Alyssa T. Reddy3, Timothy B. Mapstone2, and Dale J. Benos1

Departments of 1 Physiology and Biophysics, 2 Surgery, and 3 Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama 35294-0005

Human astrocytoma cells were studied using whole cell patch-clamp recording. An inward, amiloride-sensitive Na+ current was identified in four continuous cell lines originally derived from human glioblastoma cells (CH235, CRT, SKMG-1, and U251-MG) and in three primary cultures of cells obtained from glioblastoma multiforme tumors (up to 4 passages). In addition, cells freshly isolated from a resected medulloblastoma tumor displayed this same characteristic inward current. In contrast, amiloride-sensitive currents were not observed in normal human astrocytes, low-grade astrocytomas, or juvenile pilocytic astrocytomas. The only amiloride-sensitive Na+ channels thus far molecularly identified in brain are the brain Na+ channels (BNaCs). RT-PCR analyses demonstrated the presence of mRNA for either BNaC1 or BNaC2 in these tumors and in normal astrocytes. These results indicate that the functional expression of amiloride-sensitive Na+ currents is a characteristic feature of malignant brain tumor cells and that this pathway may be a potentially useful target for therapeutic intervention.

astrocytoma; glioblastoma; patch clamp; brain tumor cells


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