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Am J Physiol Cell Physiol 276: C1288-C1296, 1999;
0363-6143/99 $5.00
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Vol. 276, Issue 6, C1288-C1296, June 1999

Choline uptake across the ventricular membrane of neonate rat choroid plexus

Alice R. Villalobos1, Judith T. Parmelee2, and J. Larry Renfro1

1 Department of Physiology and Neurobiology, University of Connecticut, Storrs 06269; and 2 Manchester Community-Technical College, Manchester, Connecticut 06045

The uptake of [3H]choline from the cerebrospinal fluid (CSF) side of the rat neonatal choroid plexus was characterized in primary cultures of the choroidal epithelium grown on solid supports. Cell-to-medium concentration ratios were ~5 at 1 min and as high as 70 at 30 min. Apical choline uptake was facilitated; the Km was ~50 µM. Several organic cations (e.g., hemicholinium-3 and N1-methylnicotinamide) inhibited uptake. The reduction or removal of external Na+ or the addition of 5 mM LiCl had no effect on uptake. However, increasing external K+ concentration from 3 to 30 mM depolarized ventricular membrane potential (-70 to -15 mV) and reduced uptake to 45% of that for the control. Treatment with 1 mM ouabain or 2 mM BaCl2 reduced uptake 45%, and intracellular acidification reduced uptake to ~90% of that for controls. These data indicate that the uptake of choline from CSF across the ventricular membrane of the neonatal choroidal epithelium is not directly coupled to Na+ influx but is sensitive to plasma membrane electrical potential.

organic cation transport; cerebrospinal fluid-blood barrier; cholinergic metabolism


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