Am J Physiol Cell Physiol Journal of Applied Physiology
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Am J Physiol Cell Physiol 276: C1226-C1230, 1999;
0363-6143/99 $5.00
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Vol. 276, Issue 5, C1226-C1230, May 1999

RAPID COMMUNICATION
[3H]taurine and D-[3H]aspartate release from astrocyte cultures are differently regulated by tyrosine kinases

Alexander A. Mongin1,2, Jyoti M. Reddi1, Carol Charniga1,2, and Harold K. Kimelberg1,2,3

1 Division of Neurosurgery, 3 Department of Pharmacology and Neuroscience, and 2 Neuropharmacology and Neuroscience Research Group, Albany Medical College, Albany, New York 12208

Volume-dependent anion channels permeable for Cl- and amino acids are thought to play an important role in the homeostasis of cell volume. Astrocytes are the main cell type in the mammalian brain showing volume perturbations under physiological and pathophysiological conditions. We investigated the involvement of tyrosine phosphorylation in hyposmotic medium-induced [3H]taurine and D-[3H]aspartate release from primary astrocyte cultures. The tyrosine kinase inhibitors tyrphostin 23 and tyrphostin A51 partially suppressed the volume-dependent release of [3H]taurine in a dose-dependent manner with half-maximal effects at ~40 and 1 µM, respectively. In contrast, the release of D-[3H]aspartate was not significantly affected by these agents in the same concentration range. The inactive analog tyrphostin 1 had no significant effect on the release of both amino acids. The data obtained suggest the existence of at least two volume-dependent anion channels permeable to amino acids in astrocyte cultures. One of these channels is permeable to taurine and is under the control of tyrosine kinase(s). The other is permeable to both taurine and aspartate, but its volume-dependent regulation does not require tyrosine phosphorylation.

regulatory volume decrease; swelling-activated anion channels; excitatory amino acids; tyrosine phosphorylation; tyrphostins


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