Am J Physiol Cell Physiol AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 276: C1154-C1161, 1999;
0363-6143/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Spindler, B.
Right arrow Articles by Verrey, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spindler, B.
Right arrow Articles by Verrey, F.
Vol. 276, Issue 5, C1154-C1161, May 1999

Aldosterone action: induction of p21ras and fra-2 and transcription-independent decrease in myc, jun, and fos

Benjamin Spindler and François Verrey

Institute of Physiology, University of Zürich, CH-8057 Zürich, Switzerland

Adrenal steroids induce an increase in transcellular Na+ reabsorption across Xenopus laevis A6 cell epithelia that requires the action of transcriptionally regulated gene products. In a previous study we identified K-ras2 as an aldosterone-upregulated mRNA in A6 epithelia. Here, we show that in vivo injection of aldosterone in Xenopus (2.5 h) increases K-ras2 mRNA specifically in the kidney (2.5-fold) and that in A6 epithelia aldosterone (2.5 h) increases Ras protein synthesis (~6-fold). Xl-ras, another ras mRNA expressed at a low level in A6 cells, was also induced (2-fold). Aldosterone was shown to regulate the mRNA levels of several transcription factors as well. After 2 h of aldosterone treatment, fra-2 mRNA was upregulated by 130%, whereas c-myc, c-jun, c-fos, and glucocorticoid receptor mRNAs were downregulated by 23-43%. After 16 h, c-fos and GR mRNAs were further decreased, whereas levels of fra-2, c-jun, and c-myc began to return to control levels. Interestingly, the downregulation of the protooncogene mRNAs was independent of transcription. These results support the view that aldosterone exerts complex pleiotropic transcriptional and nontranscriptional actions that involve the regulation of signaling cascade elements (i.e., K-Ras2) as well as that of transcription factors.

epithelial sodium transport; K-Ras; glucocorticoid receptor; mineralocorticoid receptor; messenger ribonucleic acid stability


This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
O. Pochynyuk, J. D. Stockand, and A. Staruschenko
Ion Channel Regulation by Ras, Rho, and Rab Small GTPases
Experimental Biology and Medicine, November 1, 2007; 232(10): 1258 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
D. W. Good
Nongenomic Actions of Aldosterone on the Renal Tubule
Hypertension, April 1, 2007; 49(4): 728 - 739.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. Pochynyuk, Q. Tong, A. Staruschenko, H.-P. Ma, and J. D. Stockand
Regulation of the epithelial Na+ channel (ENaC) by phosphatidylinositides
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F949 - F957.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. N. Helms, X.-J. Chen, S. Ramosevac, D. C. Eaton, and L. Jain
Dopamine regulation of amiloride-sensitive sodium channels in lung cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L710 - L722.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W. Zhang, X. Xia, D. I. Jalal, T. Kuncewicz, W. Xu, G. D. Lesage, and B. C. Kone
Aldosterone-sensitive repression of ENaC{alpha} transcription by a histone H3 lysine-79 methyltransferase
Am J Physiol Cell Physiol, March 1, 2006; 290(3): C936 - C946.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Q. Tong, R. E. Booth, R. T. Worrell, and J. D. Stockand
Regulation of Na+ transport by aldosterone: signaling convergence and cross talk between the PI3-K and MAPK1/2 cascades
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1232 - F1238.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. D. Stockand and J. G. Meszaros
Aldosterone stimulates proliferation of cardiac fibroblasts by activating Ki-RasA and MAPK1/2 signaling
Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H176 - H184.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. Hendron and J. D. Stockand
Activation of Mitogen-activated Protein Kinase (Mitogen-activated Protein Kinase/Extracellular Signal-regulated Kinase) Cascade by Aldosterone
Mol. Biol. Cell, September 1, 2002; 13(9): 3042 - 3054.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. D. Stockand
New ideas about aldosterone signaling in epithelia
Am J Physiol Renal Physiol, April 1, 2002; 282(4): F559 - F576.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Le Menuet, R. Isnard, M. Bichara, S. Viengchareun, M. Muffat-Joly, F. Walker, M.-C. Zennaro, and M. Lombes
Alteration of Cardiac and Renal Functions in Transgenic Mice Overexpressing Human Mineralocorticoid Receptor
J. Biol. Chem., October 12, 2001; 276(42): 38911 - 38920.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
F. Verrey
Early aldosterone action: toward filling the gap between transcription and transport
Am J Physiol Renal Physiol, September 1, 1999; 277(3): F319 - F327.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online