Am J Physiol Cell Physiol AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 276: C1115-C1120, 1999;
0363-6143/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dreja, K.
Right arrow Articles by Hellstrand, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dreja, K.
Right arrow Articles by Hellstrand, P.
Vol. 276, Issue 5, C1115-C1120, May 1999

Differential modulation of caffeine- and IP3-induced calcium release in cultured arterial tissue

Karl Dreja and Per Hellstrand

Department of Physiological Sciences, Lund University, S-223 62 Lund, Sweden

To investigate the Ca2+-dependent plasticity of sarcoplasmic reticulum (SR) function in vascular smooth muscle, transient responses to agents releasing intracellular Ca2+ by either ryanodine (caffeine) or D-myo-inositol 1,4,5-trisphosphate [IP3; produced in response to norepinephrine (NE), 5-hydroxytryptamine (5-HT), arginine vasopressin (AVP)] receptors in rat tail arterial rings were evaluated after 4 days of organ culture. Force transients induced by all agents were increased compared with those induced in fresh rings. Stimulation by 10% FCS during culture further potentiated the force and Ca2+ responses to caffeine (20 mM) but not to NE (10 µM), 5-HT (10 µM), or AVP (0.1 µM). The effect was persistent, and SR capacity was not altered after reversible depletion of stores with cyclopiazonic acid. The effects of serum could be mimicked by culture in depolarizing medium (30 mM K+) and blocked by the addition of verapamil (1 µM) or EGTA (1 mM) to the medium, lowering intracellular Ca2+ concentration ([Ca2+]i) during culture. These results show that modulation of SR function can occur in vitro by a mechanism dependent on long-term levels of basal [Ca2+]i and involving ryanodine- but not IP3 receptor-mediated Ca2+ release.

sarcoplasmic reticulum; organ culture; tail artery; D-myo-inositol 1,4,5-trisphosphate


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Berra-Romani, A. Mazzocco-Spezzia, M. V. Pulina, and V. A. Golovina
Ca2+ handling is altered when arterial myocytes progress from a contractile to a proliferative phenotype in culture
Am J Physiol Cell Physiol, September 1, 2008; 295(3): C779 - C790.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Bergdahl, M. F. Gomez, A.-K. Wihlborg, D. Erlinge, A. Eyjolfson, S.-Z. Xu, D. J. Beech, K. Dreja, and P. Hellstrand
Plasticity of TRPC expression in arterial smooth muscle: correlation with store-operated Ca2+ entry
Am J Physiol Cell Physiol, April 1, 2005; 288(4): C872 - C880.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. D. Thorne and R. J. Paul
Effects of organ culture on arterial gene expression and hypoxic relaxation: role of the ryanodine receptor
Am J Physiol Cell Physiol, April 1, 2003; 284(4): C999 - C1005.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Lindqvist, K. Dreja, K. Sward, and P. Hellstrand
Effects of oxygen tension on energetics of cultured vascular smooth muscle
Am J Physiol Heart Circ Physiol, July 1, 2002; 283(1): H110 - H117.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Lindqvist, I. Nordstrom, U. Malmqvist, P. Nordenfelt, and P. Hellstrand
Long-term effects of Ca2+ on structure and contractility of vascular smooth muscle
Am J Physiol Cell Physiol, July 1, 1999; 277(1): C64 - C73.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online