Am J Physiol Cell Physiol Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 276: C1038-C1045, 1999;
0363-6143/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prakash, Y. S.
Right arrow Articles by Sieck, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prakash, Y. S.
Right arrow Articles by Sieck, G. C.
Vol. 276, Issue 5, C1038-C1045, May 1999

Effect of beta -adrenoceptor activation on [Ca2+]i regulation in murine skeletal myotubes

Y. S. Prakash1, H. F. M. van der Heijden2, E. M. Gallant3, and G. C. Sieck1

1 Departments of Anesthesiology and of Physiology and Biophysics, Mayo Clinic and Foundation, Rochester 55905; 3 Department of Veterinary Pathobiology, University of Minnesota, St. Paul, Minnesota 55108; and 2 Department of Pulmonary Diseases, University Hospital Nijmegen, 6500HB Nijmegen, The Netherlands

The present study used real-time confocal microscopy to examine the effects of the beta 2-adrenoceptor agonist salbutamol on regulation of intracellular Ca2+ concentration ([Ca2+]i) in myotubes derived from neonatal mouse limb muscles. Immunocytochemical staining for ryanodine receptors and skeletal muscle myosin confirmed the presence of sarcomeres. The myotubes displayed both spontaneous and ACh-induced rapid (<2-ms rise time) [Ca2+]i transients. The [Ca2+]i transients were frequency modulated by both low and high concentrations of salbutamol. Exposure to alpha -bungarotoxin and tetrodotoxin inhibited ACh-induced [Ca2+]i transients and the response to low concentrations of salbutamol but not the response to higher concentrations. Preexposure to caffeine inhibited the subsequent [Ca2+]i response to lower concentrations of salbutamol and significantly blunted the response to higher concentrations. Preexposure to salbutamol diminished the [Ca2+]i response to caffeine. Inhibition of dihydropyridine-sensitive Ca2+ channels with nifedipine or PN-200-110 did not prevent [Ca2+]i elevations induced by higher concentrations of salbutamol. The effects of salbutamol were mimicked by the membrane-permeant analog dibutyryl adenosine 3',5'-cyclic monophosphate. These data indicate that salbutamol effects in skeletal muscle predominantly involve enhanced sarcoplasmic reticulum Ca2+ release.

adenosine 3',5'-cyclic monophosphate; ryanodine receptor; sarcoplasmic reticulum; skeletal muscle


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. Polla, V. Cappelli, F. Morello, M. A. Pellegrino, F. Boschi, O. Pastoris, and C. Reggiani
Effects of the {beta}2-agonist clenbuterol on respiratory and limb muscles of weaning rats
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2001; 280(3): R862 - R869.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
P N R Dekhuijzen, H A Machiels, L M A Heunks, H F M van der Heijden, and R H H van Balkom
Athletes and doping: effects of drugs on the respiratory system
Thorax, November 1, 1999; 54(11): 1041 - 1046.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online