Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 276: C865-C872, 1999;
0363-6143/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dall'Asta, V.
Right arrow Articles by Gazzola, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dall'Asta, V.
Right arrow Articles by Gazzola, G. C.
Vol. 276, Issue 4, C865-C872, April 1999

Amino acids are compatible osmolytes for volume recovery after hypertonic shrinkage in vascular endothelial cells

Valeria Dall'Asta1, Ovidio Bussolati1, Roberto Sala1, Alessandro Parolari2, Francesco Alamanni2, Paolo Biglioli2, and Gian C. Gazzola1

1 Istituto di Patologia Generale, Università degli Studi di Parma, 43100 Parma; and 2 Dipartimento di Cardiochirurgia, Università degli Studi di Milano, Centro Cardiologico Fondazione Monzino Istituto di Ricovero e Cura a Carattere Scientifico, 20122 Milan, Italy

The response to chronic hypertonic stress has been studied in human endothelial cells derived from saphenous veins. In complete growth medium the full recovery of cell volume requires several hours and is neither associated with an increase in cell K+ nor hindered by bumetanide but depends on an increased intracellular pool of amino acids. The highest increase is exhibited by neutral amino acid substrates of transport system A, such as glutamine and proline, and by the anionic amino acid glutamate. Transport system A is markedly stimulated on hypertonic stress, with an increase in activity roughly proportional to the extent and the duration of the osmotic shrinkage. Cycloheximide prevents the increase in transport activity of system A and the recovery of cell volume. It is concluded that human endothelial cells counteract hypertonic stress through the stimulation of transport system A and the consequent expansion of the intracellular amino acid pool.

glutamine; system A; amino acid transport; regulatory volume increase; bumetanide


This article has been cited by other articles:


Home page
 J. Physiol.Home page
H. Zhao, R. Hyde, and H. S Hundal
Signalling mechanisms underlying the rapid and additive stimulation of NKCC activity by insulin and hypertonicity in rat L6 skeletal muscle cells
J. Physiol., October 1, 2004; 560(1): 123 - 136.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. E. Mann, D. L. Yudilevich, and L. Sobrevia
Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells
Physiol Rev, January 1, 2003; 83(1): 183 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Sala, B. M. Rotoli, E. Colla, R. Visigalli, A. Parolari, O. Bussolati, G. C. Gazzola, and V. Dall'Asta
Two-way arginine transport in human endothelial cells: TNF-alpha stimulation is restricted to system y+
Am J Physiol Cell Physiol, January 1, 2002; 282(1): C134 - C143.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Franchi-Gazzola, R. Visigalli, V. Dall'Asta, R. Sala, S. K. Woo, H. M. Kwon, G. C. Gazzola, and O. Bussolati
Amino acid depletion activates TonEBP and sodium-coupled inositol transport
Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1465 - C1474.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
V. Dall'Asta, O. Bussolati, R. Sala, B. M. Rotoli, G. Sebastio, M. P. Sperandeo, G. Andria, and G. C. Gazzola
Arginine transport through system y+L in cultured human fibroblasts: normal phenotype of cells from LPI subjects
Am J Physiol Cell Physiol, December 1, 2000; 279(6): C1829 - C1837.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P.-G. Petronini, R. R. Alfieri, M. N. Losio, A. E. Caccamo, A. Cavazzoni, M. A. Bonelli, A. F. Borghetti, and K. P. Wheeler
Induction of BGT-1 and amino acid System A transport activities in endothelial cells exposed to hyperosmolarity
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2000; 279(5): R1580 - R1589.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Franchi-Gazzola, R. Visigalli, O. Bussolati, V. Dall'Asta, and G. C. Gazzola
Adaptive Increase of Amino Acid Transport System A Requires ERK1/2 Activation
J. Biol. Chem., October 8, 1999; 274(41): 28922 - 28928.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online