Am J Physiol Cell Physiol Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 276: C593-C601, 1999;
0363-6143/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Obeso, A.
Right arrow Articles by Gonzalez, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Obeso, A.
Right arrow Articles by Gonzalez, C.
Vol. 276, Issue 3, C593-C601, March 1999

NADPH oxidase inhibition does not interfere with low PO2 transduction in rat and rabbit CB chemoreceptor cells

A. Obeso, A. Gómez-Niño, and C. Gonzalez

Departamento de Bioquímica y Biología Molecular y Fisiología, Instituto de Biología y Genética Molecular, Consejo Superior Investigaciones Científicas, Facultad de Medicina, Universidad de Valladolid, 47005 Valladolid, Spain

The aim of the present work was to elucidate the role of NADPH oxidase in hypoxia sensing and transduction in the carotid body (CB) chemoreceptor cells. We have studied the effects of several inhibitors of NADPH oxidase on the normoxic and hypoxia-induced release of [3H]catecholamines (CA) in an in vitro preparation of intact CB of the rat and rabbit whose CA deposits have been labeled by prior incubation with the natural precursor [3H]tyrosine. It was found that diphenyleneiodonium (DPI; 0.2-25 µM), an inhibitor of NADPH oxidase, caused a dose-dependent release of [3H]CA from normoxic CB chemoreceptor cells. Contrary to hypoxia, DPI-evoked release was only partially Ca2+ dependent. Concentrations of DPI reported to produce full inhibition of NADPH oxidase in the rat CB did not prevent the hypoxic release response in the rat and rabbit CB chemoreceptor cells, as stimulation with hypoxia in the presence of DPI elicited a response equaling the sum of that produced by DPI and hypoxia applied separately. Neopterin (3-300 µM) and phenylarsine oxide (0.5-2 µM), other inhibitors of NADPH oxidase, did not promote release of [3H]CA in normoxic conditions or affect the response elicited by hypoxia. On the basis of effects of neopterin and phenylarsine oxide, it is concluded that NADPH oxidase does not appear to play a role in oxygen sensing or transduction in the rat and rabbit CB chemoreceptor cells in vitro and, in the context of the present study, that DPI effects are not related to NADPH oxidase inhibition.

hypoxia; reactive oxygen species; diphenyleneiodonium; neopterin; phenylarsine oxide; carotid body; partial pressure of oxygen


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. He, B. Dinger, K. Sanders, J. Hoidal, A. Obeso, L. Stensaas, S. Fidone, and C. Gonzalez
Effect of p47phox gene deletion on ROS production and oxygen sensing in mouse carotid body chemoreceptor cells
Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L916 - L924.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. J. Kemp, A. Lewis, M. E. Hartness, G. J. Searle, P. Miller, I. O'Kelly, and C. Peers
Airway Chemotransduction: From Oxygen Sensor to Cellular Effector
Am. J. Respir. Crit. Care Med., December 15, 2002; 166(12): S17 - 24.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. A. Sanders, K. M. Sundar, L. He, B. Dinger, S. Fidone, and J. R. Hoidal
Role of components of the phagocytic NADPH oxidase in oxygen sensing
J Appl Physiol, October 1, 2002; 93(4): 1357 - 1364.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. He, J. Chen, B. Dinger, K. Sanders, K. Sundar, J. Hoidal, and S. Fidone
Characteristics of carotid body chemosensitivity in NADPH oxidase-deficient mice
Am J Physiol Cell Physiol, January 1, 2002; 282(1): C27 - C33.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
N. R. Prabhakar
Oxygen sensing by the carotid body chemoreceptors
J Appl Physiol, June 1, 2000; 88(6): 2287 - 2295.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online