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Am J Physiol Cell Physiol 276: C548-C557, 1999;
0363-6143/99 $5.00
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Vol. 276, Issue 3, C548-C557, March 1999

Transport of fluid by lens epithelium

Jorge Fischbarg1,2, Friedrich P. J. Diecke3, Kunyan Kuang2, Bin Yu2, Fengying Kang2, Pavel Iserovich2, Yansui Li2, Heinz Rosskothen2, and Jan P. Koniarek2

Departments of 1 Physiology and Cellular Biophysics and 2 Ophthalmology, Columbia University, New York, New York 10032; and 3 Department of Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103

We report for the first time that cultured lens epithelial cell layers and rabbit lenses in vitro transport fluid. Layers of the alpha TN4 mouse cell line and bovine cell cultures were grown to confluence on permeable membrane inserts. Fluid movement across cultured layers and excised rabbit lenses was determined by volume clamp (37°C). Cultured layers transported fluid from their basal to their apical sides against a pressure head of 3 cmH2O. Rates were (in µl · h-1 · cm-2) 3.3 ± 0.3 for alpha TN4 cells (n = 27) and 4.7 ± 1.0 for bovine layers (n = 6). Quinidine, a blocker of K+ channels, and p-chloromercuribenzenesulfonate and HgCl2, inhibitors of aquaporins, inhibited fluid transport. Rabbit lenses transported fluid from their anterior to their posterior sides against a 2.5-cmH2O pressure head at 10.3 ± 0.62 µl · h-1 · lens-1 (n = 5) and along the same pressure head at 12.5 ± 1.1 µl · h-1 · lens-1 (n = 6). We calculate that this flow could wash the lens extracellular space by convection about once every 2 h and therefore might contribute to lens homeostasis and transparency.

cell layers; fluid circulation; lens extracellular space; lens homeostasis; nutrient transport


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