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Am J Physiol Cell Physiol 276: C312-C317, 1999;
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Vol. 276, Issue 2, C312-C317, February 1999

Oxygen modulates Na+ absorption in middle ear epithelium

F. Portier1, T. van den Abbeele1, E. Lecain1, E. Sauvaget1, B. Escoubet2, P. Tran Ba Huy1, and P. Herman1

1 Laboratoire d'Otologie Expérimentale, Faculté Lariboisière-St-Louis and 2 Department of Physiology, Institut National de la Santé et de la Recherche Médicale Unité 426, Faculté Xavier Bichat, Université Paris VII, 75010 Paris, France

The physiology of the middle ear is primarily concerned with keeping the cavities air filled and fluid free to allow transmission of the sound vibrations from the eardrum to the inner ear. Middle ear epithelial cells are thought to play a key role in this process, since they actively transport Na+ and water. The PO2 of the middle ear cavities varies from 44 to 54 mmHg in healthy human ears but may be lower in the course of secretory otitis media. The effect of chronic hypoxia on ion transport was investigated on a middle ear cell line using the short-circuit current technique. Chronic hypoxia reversibly decreased the rate of Na+ absorption across the MESV cell line. Although a decrease in cellular ATP content was observed, the decrease of Na+ absorption seemed related to a primary modulation of apical Na+ entry. As revealed by RNase protection assay, the decrease in the rate of apical Na+ entry strictly paralleled the decrease in the expression of transcripts encoding the alpha -subunit of the epithelial Na+ channel. This effect of oxygen on Na+ absorption might account for 1) the presence of fluid in the middle ear in the course of secretory otitis media and 2) the beneficial effect of the ventilation tube in treating otitis media that allows the PO2 to rise and restores the fluid clearance.

secretory otitis media; ouabain; benzamil; ion transport; transepithelial sodium; short-circuit current; epithelial sodium channel; sodium-potasium-adenosine 5'-triphosphatase


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