Am J Physiol Cell Physiol Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 276: C54-C65, 1999;
0363-6143/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gueorguiev, V. D.
Right arrow Articles by Sabban, E. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gueorguiev, V. D.
Right arrow Articles by Sabban, E. L.
Vol. 276, Issue 1, C54-C65, January 1999

Differing temporal roles of Ca2+ and cAMP in nicotine-elicited elevation of tyrosine hydroxylase mRNA

Volodia D. Gueorguiev1, Richard J. Zeman2, Bhargava Hiremagalur1, Ana Menezes1, and Esther L. Sabban1

Departments of 1 Biochemistry and Molecular Biology and 2 Cell Biology and Anatomy, New York Medical College, Valhalla, New York 10595

The involvement of cAMP- and Ca2+-mediated pathways in the activation of tyrosine hydroxylase (TH) gene expression by nicotine was examined in PC-12 cells. Extracellular Ca2+ and elevations in intracellular Ca2+ concentration ([Ca2+]i) were required for nicotine to increase TH mRNA. The nicotine-elicited rapid rise in [Ca2+]i was inhibited by blockers of either L-type or N-type, and to a lesser extent P/Q-, but not T-type, voltage-gated Ca2+ channels. With continual nicotine treatment, [Ca2+]i returned to basal levels within 3-4 min. After a lag of ~5-10 min, there was a smaller elevation in [Ca2+]i that persisted for 6 h and displayed different responsiveness to Ca2+ channel blockers. This second phase of elevated [Ca2+]i was blocked by an inhibitor of store-operated Ca2+ channels, consistent with the observed generation of inositol trisphosphate. 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-AM (BAPTA-AM), when added before or 2 h after nicotine, prevented elevation of TH mRNA. Nicotine treatment significantly raised cAMP levels. Addition of the adenylyl cyclase inhibitor 2',5'-dideoxyadenosine (DDA) prevented the nicotine-elicited phosphorylation of cAMP response element binding protein. DDA also blocked the elevation of TH mRNA only when added after the initial transient rise in [Ca2+]i and not after 1 h. This study reveals that several temporal phases are involved in the induction of TH gene expression by nicotine, each of them with differing requirements for Ca2+ and cAMP.

adenylyl cyclase; voltage-gated calcium channels; adenosine 3',5'-cyclic monophosphate response element binding protein


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Oshikawa, Y. Toya, T. Fujita, M. Egawa, J. Kawabe, S. Umemura, and Y. Ishikawa
Nicotinic acetylcholine receptor {alpha}7 regulates cAMP signal within lipid rafts
Am J Physiol Cell Physiol, September 1, 2003; 285(3): C567 - C574.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
B. J. Dewar, B. U. Bradford, and R. G. Thurman
Nicotine Increases Hepatic Oxygen Uptake in the Isolated Perfused Rat Liver by Inhibiting Glycolysis
J. Pharmacol. Exp. Ther., June 1, 2002; 301(3): 930 - 937.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
E. B. Gauda, R. Cooper, P. K. Akins, and G. Wu
Prenatal nicotine affects catecholamine gene expression in newborn rat carotid body and petrosal ganglion
J Appl Physiol, November 1, 2001; 91(5): 2157 - 2165.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. Venkatesh, G. Siddhartha, R. Joshi, S. Patel, and G. Hasan
Interactions Between the Inositol 1,4,5-Trisphosphate and Cyclic AMP Signaling Pathways Regulate Larval Molting in Drosophila
Genetics, May 1, 2001; 158(1): 309 - 318.
[Abstract] [Full Text]

Home page
 Mol Hum ReprodHome page
J. B.Mailhes, D. Young, G. Caldito, and S.N. London
Sensitivity of mouse oocytes to nicotine-induced perturbations during oocyte meiotic maturation and aneuploidy in vivo and in vitro
Mol. Hum. Reprod., March 1, 2000; 6(3): 232 - 237.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online