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Am J Physiol Cell Physiol 276: C238-C249, 1999;
0363-6143/99 $5.00
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Vol. 276, Issue 1, C238-C249, January 1999

Mechanical stress-induced Ca2+ entry and Clminus current in cultured human aortic endothelial cells

Miki Nakao, Kyoichi Ono, Susumu Fujisawa, and Toshihiko Iijima

Department of Pharmacology, Akita University School of Medicine, Akita 010-8543, Japan

A fluid stream through a microtube was applied to cultured human aortic endothelial cells to investigate the endothelial responses of both the ionic currents and intracellular Ca2+ concentration ([Ca2+]i) to mechanical stimulation. The fluid stream induced an increase in [Ca2+]i that was dependent on both the flow rate and the extracellular Ca2+ concentration. Gd3+ and niflumic acid inhibited the fluid stream-induced increase in [Ca2+]i, whereas Ba2+ and tetraethylammonium ion exhibited no effect. The fluid stream-induced [Ca2+]i increase was accompanied by the activation of an inward current at -52.8 mV. The reversal potential of the fluid stream-induced current shifted to positive potentials when the external Cl- concentration was reduced but was not affected by variation of the external Na+ concentration. During the exposure to the fluid stream, [Ca2+]i was voltage dependent, i.e., depolarization decreased [Ca2+]i. We therefore conclude that the fluid stream-induced current is largely carried by Cl- and that the Cl- current may thus play a role in modulating the Ca2+ influx by altering the membrane potential of endothelial cells.

shear stress; stretch; calcium signaling


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