| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Pulmonary and Critical Care
Division,
Reactive oxygen species (ROS) play an important role in the pathogenesis of many human diseases, including the acute respiratory distress syndrome, Parkinson's disease, pulmonary fibrosis, and Alzheimer's disease. In mammalian cells, several genes known to be induced during the immediate early response to growth factors, including the protooncogenes c-fos and c-myc, have also been shown to be induced by ROS. We show that members of the STAT family of transcription factors, including STAT1 and STAT3, are activated in fibroblasts and A-431 carcinoma cells in response to H2O2. This activation occurs within 5 min, can be inhibited by antioxidants, and does not require protein synthesis. STAT activation in these cell lines is oxidant specific and does not occur in response to superoxide- or nitric oxide-generating stimuli. Buthionine sulfoximine, which depletes intracellular glutathione, also activates the STAT pathway. Moreover, H2O2 stimulates the activity of the known STAT kinases JAK2 and TYK2. Activation of STATs by platelet-derived growth factor (PDGF) is significantly inhibited by N-acetyl-L-cysteine and diphenylene iodonium, indicating that ROS production contributes to STAT activation in response to PDGF. These findings indicate that the JAK-STAT pathway responds to intracellular ROS and that PDGF uses ROS as a second messenger to regulate STAT activation.
hydrogen peroxide; platelet-derived growth factor; TYK2
This article has been cited by other articles:
|
|
 |
|
  G. Byfield, S. Budd, and M. E. Hartnett The Role of Supplemental Oxygen and JAK/STAT Signaling in Intravitreous Neovascularization in a ROP Rat Model Invest. Ophthalmol. Vis. Sci., July 1, 2009; 50(7): 3360 - 3365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Simeone-Penney, M. Severgnini, L. Rozo, S. Takahashi, B. H. Cochran, and A. R. Simon PDGF-induced human airway smooth muscle cell proliferation requires STAT3 and the small GTPase Rac1 Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L698 - L704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cao, T. L. Tal, L. M. Graves, I. Gilmour, W. Linak, W. Reed, P. A. Bromberg, and J. M. Samet Diesel exhaust particulate-induced activation of Stat3 requires activities of EGFR and Src in airway epithelial cells Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L422 - L429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Marrero, A. K. Banes-Berceli, D. M. Stern, and D. C. Eaton Role of the JAK/STAT signaling pathway in diabetic nephropathy Am J Physiol Renal Physiol, April 1, 2006; 290(4): F762 - F768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Stocker and J. F. Keaney Jr. Role of Oxidative Modifications in Atherosclerosis Physiol Rev, October 1, 2004; 84(4): 1381 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zanetti, Z. S. Katusic, and T. O'Brien Adenoviral-mediated overexpression of catalase inhibits endothelial cell proliferation Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2620 - H2626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, E. L. Bedard, R. Potter, R. Zhong, J. Alam, A. M. K. Choi, and P. J. Lee Mitogen-activated protein kinases regulate HO-1 gene transcription after ischemia-reperfusion lung injury Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L815 - L829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. A. Comhair and S. C. Erzurum Antioxidant responses to oxidant-mediated lung diseases Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L246 - L255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Carcamo, O. Borquez-Ojeda, and D. W. Golde Vitamin C inhibits granulocyte macrophage-colony-stimulating factor-induced signaling pathways Blood, May 1, 2002; 99(9): 3205 - 3212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lum and K. A. Roebuck Oxidant stress and endothelial cell dysfunction Am J Physiol Cell Physiol, April 1, 2001; 280(4): C719 - C741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. HERRERA, ALBERTO. M. ALVAREZ, A. SANCHEZ, M. FERNANDEZ, C. RONCERO, M. BENITO, and I. FABREGAT Reactive oxygen species (ROS) mediates the mitochondrial-dependent apoptosis induced by transforming growth factor {beta} in fetal hepatocytes FASEB J, March 1, 2001; 15(3): 741 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cuzzocrea, D. P. Riley, A. P. Caputi, and D. Salvemini Antioxidant Therapy: A New Pharmacological Approach in Shock, Inflammation, and Ischemia/Reperfusion Injury Pharmacol. Rev., March 1, 2001; 53(1): 135 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schieffer, M. Luchtefeld, S. Braun, A. Hilfiker, D. Hilfiker-Kleiner, and H. Drexler Role of NAD(P)H Oxidase in Angiotensin II-Induced JAK/STAT Signaling and Cytokine Induction Circ. Res., December 8, 2000; 87(12): 1195 - 1201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. J. Thannickal and B. L. Fanburg Reactive oxygen species in cell signaling Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1005 - L1028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Lee, S. L. Camhi, B. Y. Chin, J. Alam, and A. M. K. Choi AP-1 and STAT mediate hyperoxia-induced gene transcription of heme oxygenase-1 Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L175 - L182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Ali, S. A. Schlidt, N. S. Chandel, K. L. Hynes, P. T. Schumacker, and B. L. Gewertz Endothelial permeability and IL-6 production during hypoxia: role of ROS in signal transduction Am J Physiol Lung Cell Mol Physiol, November 1, 1999; 277(5): L1057 - L1065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Griendling and D. G. Harrison Dual Role of Reactive Oxygen Species in Vascular Growth Circ. Res., September 17, 1999; 85(6): 562 - 563. [Full Text] [PDF]
|
|
|
|
|
|
A. R. Simon, S. Takahashi, M. Severgnini, B. L. Fanburg, and B. H. Cochran Role of the JAK-STAT pathway in PDGF-stimulated proliferation of human airway smooth muscle cells Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1296 - L1304. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
