| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
channels
Departments of Physiology and Medicine, University of Minnesota, Minneapolis, Minnesota 55455
Alveolar epithelial cells were isolated from adult
Sprague-Dawley rats and grown to confluence on membrane filters. Most
of the basal short-circuit current
(Isc; 60%) was
inhibited by amiloride (IC50 0.96 µM) or benzamil (IC50 0.5 µM).
Basolateral addition of terbutaline (2 µM) produced a rapid decrease
in Isc, followed by a slow recovery back to its initial amplitude. When
Cl
was replaced with
methanesulfonic acid, the basal
Isc was reduced and the response to terbutaline was inhibited. In permeabilized monolayer experiments, both terbutaline and amiloride produced sustained decreases in current. The current-voltage relationship of the terbutaline-sensitive current had a reversal potential of
28 mV. Increasing Cl concentration in the
basolateral solution shifted the reversal potential to more depolarized
voltages. These results were consistent with the existence of a
terbutaline-activated Cl conductance in the apical
membrane. Terbutaline did not increase the amiloride-sensitive
Na+ conductance. We conclude that 
-adrenergic
stimulation of adult alveolar epithelial cells results in an increase
in apical Cl permeability and that
amiloride-sensitive Na+ channels are not directly affected
by this stimulation.
chloride absorption; cystic fibrosis transmembrane conductance regulator; sodium channel; glibenclamide; amiloride; alveolar type II cells; ion transport
This article has been cited by other articles:
|
|
 |
|
  M. Johnson, L. Allen, and L. Dobbs Characteristics of Cl- uptake in rat alveolar type I cells Am J Physiol Lung Cell Mol Physiol, November 1, 2009; 297(5): L816 - L827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Ingbar, M. Bhargava, and S. M. O'Grady Mechanisms of alveolar epithelial chloride absorption Am J Physiol Lung Cell Mol Physiol, November 1, 2009; 297(5): L813 - L815. [Full Text] [PDF]
|
|
|
|
|
|
C. Yang, L. Su, Y. Wang, and L. Liu UTP regulation of ion transport in alveolar epithelial cells involves distinct mechanisms Am J Physiol Lung Cell Mol Physiol, September 1, 2009; 297(3): L439 - L454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. DeMaio, W. Tseng, Z. Balverde, J. R. Alvarez, K.-J. Kim, D. G. Kelley, R. M. Senior, E. D. Crandall, and Z. Borok Characterization of mouse alveolar epithelial cell monolayers Am J Physiol Lung Cell Mol Physiol, June 1, 2009; 296(6): L1051 - L1058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Randrianarison, B. Escoubet, C. Ferreira, A. Fontayne, N. Fowler-Jaeger, C. Clerici, E. Hummler, B. C. Rossier, and C. Planes beta-Liddle mutation of the epithelial sodium channel increases alveolar fluid clearance and reduces the severity of hydrostatic pulmonary oedema in mice J. Physiol., July 15, 2007; 582(2): 777 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yasuda, N. Niisato, H. Miyazaki, T. Hama, K. Dejima, Y. Hisa, and Y. Marunaka Epithelial Ion Transport of Human Nasal Polyp and Paranasal Sinus Mucosa Am. J. Respir. Cell Mol. Biol., April 1, 2007; 36(4): 466 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lei, C. H. Wendt, D. Fan, C. N. Mariash, and D. H. Ingbar Developmental acquisition of T3-sensitive Na-K-ATPase stimulation by rat alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L6 - L14. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Jain and D. C. Eaton Alveolar fluid transport: a changing paradigm Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L646 - L648. [Full Text] [PDF]
|
|
|
|
|
|
T. Li and H. G. Folkesson RNA interference for {alpha}-ENaC inhibits rat lung fluid absorption in vivo Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L649 - L660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fang, Y. Song, J. Hirsch, L. J. V. Galietta, N. Pedemonte, R. L. Zemans, G. Dolganov, A. S. Verkman, and M. A. Matthay Contribution of CFTR to apical-basolateral fluid transport in cultured human alveolar epithelial type II cells Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L242 - L249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Mutlu and J. I. Sznajder Mechanisms of pulmonary edema clearance Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L685 - L695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Matthay, L. Robriquet, and X. Fang Alveolar Epithelium: Role in Lung Fluid Balance and Acute Lung Injury Proceedings of the ATS, October 1, 2005; 2(3): 206 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Thomas, J. R. Campbell, P. J. Wright, and R. F. Husted cAMP-stimulated Na+ transport in H441 distal lung epithelial cells: role of PKA, phosphatidylinositol 3-kinase, and sgk1 Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L843 - L851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Kemp and K.-J. Kim Spectrum of ion channels in alveolar epithelial cells: implications for alveolar fluid balance Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L460 - L464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Brochiero, A. Dagenais, A. Prive, Y. Berthiaume, and R. Grygorczyk Evidence of a functional CFTR Cl- channel in adult alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L382 - L392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Ramminger, K. Richard, S. K. Inglis, S. C. Land, R. E. Olver, and S. M. Wilson A regulated apical Na+ conductance in dexamethasone-treated H441 airway epithelial cells Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L411 - L419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Berthiaume Long-term stimulation of alveolar epithelial cells by {beta}-adrenergic agonists: increased Na+ transport and modulation of cell growth? Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L798 - L801. [Full Text] [PDF]
|
|
|
|
|
|
A. Lazrak and S. Matalon cAMP-induced changes of apical membrane potentials of confluent H441 monolayers Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L443 - L450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Lee, P. J. Maniak, D. H. Ingbar, and S. M. O'Grady Adult alveolar epithelial cells express multiple subtypes of voltage-gated K+ channels that are located in apical membrane Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1614 - C1624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. O'Grady and S. Y. Lee Chloride and potassium channel function in alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L689 - L700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jiang, Y. Song, C. Bai, B. H. Koller, M. A. Matthay, and A. S. Verkman Pleural surface fluorescence measurement of Na+ and Cl- transport across the air space-capillary barrier J Appl Physiol, January 1, 2003; 94(1): 343 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Matalon, A. Lazrak, L. Jain, and D. C. Eaton Lung Edema Clearance: 20 Years of Progress: Invited Review: Biophysical properties of sodium channels in lung alveolar epithelial cells J Appl Physiol, November 1, 2002; 93(5): 1852 - 1859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. I. Sznajder, P. Factor, and D. H. Ingbar Lung Edema Clearance: 20 Years of Progress: Invited Review: Lung edema clearance: role of Na+-K+-ATPase J Appl Physiol, November 1, 2002; 93(5): 1860 - 1866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Crandall and R. M. Effros Lung Edema Clearance: 20 Years of Progress: Historical perspectives on lung edema clearance J Appl Physiol, October 1, 2002; 93(4): 1527 - 1532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Matthay, C. Clerici, and G. Saumon Lung Edema Clearance: 20 Years of Progress: Invited Review: Active fluid clearance from the distal air spaces of the lung J Appl Physiol, October 1, 2002; 93(4): 1533 - 1541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Matthay, H. G. Folkesson, and C. Clerici Lung Epithelial Fluid Transport and the Resolution of Pulmonary Edema Physiol Rev, July 1, 2002; 82(3): 569 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Hardiman and S. Matalon Modification of Sodium Transport and Alveolar Fluid Clearance by Hypoxia . Mechanisms and Physiological Implications Am. J. Respir. Cell Mol. Biol., November 1, 2001; 25(5): 538 - 541. [Full Text] [PDF]
|
|
|
|
|
|
A. Norlin, L. N. Lu, S. E. Guggino, M. A. Matthay, and H. G. Folkesson Contribution of amiloride-insensitive pathways to alveolar fluid clearance in adult rats J Appl Physiol, April 1, 2001; 90(4): 1489 - 1496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. CRANDALL and M. A. MATTHAY Alveolar Epithelial Transport . Basic Science to Clinical Medicine Am. J. Respir. Crit. Care Med., March 15, 2001; 163(4): 1021 - 1029. [Full Text]
|
|
|
|
|
|
X. Jiang, D. H. Ingbar, and S. M. O'Grady Selectivity properties of a Na-dependent amino acid cotransport system in adult alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, November 1, 2000; 279(5): L911 - L915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Frank, Y. Wang, O. Osorio, and M. A. Matthay beta -Adrenergic agonist therapy accelerates the resolution of hydrostatic pulmonary edema in sheep and rats J Appl Physiol, October 1, 2000; 89(4): 1255 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Widdcombe How does cAMP increase active Na absorption across alveolar epithelium? Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L231 - L232. [Full Text] [PDF]
|
|
|
|
|
|
A. Lazrak, V. G. Nielsen, and S. Matalon Mechanisms of increased Na+ transport in ATII cells by cAMP: we agree to disagree and do more experiments Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L233 - L238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. O'Grady, X. Jiang, and D. H. Ingbar Cl-channel activation is necessary for stimulation of Na transport in adult alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L239 - L244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. DeCoursey Hypothesis: do voltage-gated H+ channels in alveolar epithelial cells contribute to CO2 elimination by the lung? Am J Physiol Cell Physiol, January 1, 2000; 278(1): C1 - C10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Collett, S. J. Ramminger, R. E. Olver, and S. M. Wilson Alveolar Epithelial Ion and Fluid Transport: beta -Adrenoceptor-mediated control of apical membrane conductive properties in fetal distal lung epithelia Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L621 - L630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-J. Chen, D. C. Eaton, and L. Jain Alveolar Epithelial Ion and Fluid Transport: beta -Adrenergic regulation of amiloride-sensitive lung sodium channels Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L609 - L620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lazrak, U. Thome, C. Myles, J. Ware, L. Chen, C. J. Venglarik, and S. Matalon Alveolar Epithelial Ion and Fluid Transport: cAMP regulation of Cl- and HCO3- secretion across rat fetal distal lung epithelial cells Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L650 - L658. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
