Am J Physiol Cell Physiol Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 275: C1277-C1283, 1998;
0363-6143/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wilding, M.
Right arrow Articles by Dale, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilding, M.
Right arrow Articles by Dale, B.
Vol. 275, Issue 5, C1277-C1283, November 1998

ADP-ribose gates the fertilization channel in ascidian oocytes

Martin Wilding1, Gian Luigi Russo2, Anthony Galione3, Marcella Marino1, and Brian Dale1

1 Stazione Zoologica "Anton Dohrn," 80121 Naples; 2 Institute of Food Science and Technology, 83100 Avellino, Italy; and 3 University Department of Pharmacology, Mansfield Road, Oxford OX1 3QT, United Kingdom

We report an ion channel in the plasma membrane of unfertilized oocytes of the ascidian Ciona intestinalis that is directly gated by the second messenger ADP-ribose. The ion channel is permeable to Ca2+ and Na+ and is characterized by a reversal potential between 0 and +20 mV and a unitary conductance of 140 pS. Preinjection of the Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) or antagonists of intracellular Ca2+ release channels into oocytes did not inhibit the ADP-ribose current, demonstrating that the channel is activated in a Ca2+-independent manner. Both the fertilization current and the current induced by the injection of nicotinamide nucleotides are blocked by nicotinamide, suggesting that the ADP-ribose channel is activated at fertilization in a nicotinamide-sensitive manner. These data suggest that ascidian sperm trigger the hydrolysis of nicotinamide nucleotides in the oocyte to ADP-ribose and that this mechanism is responsible for the production of the fertilization current.

nicotinamide nucleotides; electrophysiology


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
M. Whitaker
Calcium at Fertilization and in Early Development
Physiol Rev, January 1, 2006; 86(1): 25 - 88.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Campo, A. Surprenant, and R. A. North
Sustained Depolarization and ADP-Ribose Activate a Common Ionic Current in Rat Peritoneal Macrophages
J. Immunol., February 1, 2003; 170(3): 1167 - 1173.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
Y. Sano, K. Inamura, A. Miyake, S. Mochizuki, H. Yokoi, H. Matsushime, and K. Furuichi
Immunocyte Ca2+ Influx System Mediated by LTRPC2
Science, August 17, 2001; 293(5533): 1327 - 1330.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P.-L. Li, D. X. Zhang, Z.-D. Ge, and W. B. Campbell
Role of ADP-ribose in 11,12-EET-induced activation of KCa channels in coronary arterial smooth muscle cells
Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1229 - H1236.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online