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Am J Physiol Cell Physiol 275: C840-C847, 1998;
0363-6143/98 $5.00
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Vol. 275, Issue 3, C840-C847, September 1998

Tissue distribution of immunoreactive mouse extracellular superoxide dismutase

Tomomi Ookawara1, Nobuo Imazeki2, Osamu Matsubara2, Takako Kizaki1, Shuji Oh-Ishi1, Chitose Nakao3, Yuzo Sato3, and Hideki Ohno1

Departments of 1 Hygiene and 2 Pathology II, National Defense Medical College, Tokorozawa, Saitama 359-8513; and 3 Research Center of Health, Physical Fitness, and Sports, Nagoya University, Nagoya, Aichi 464-01, Japan

Protein content and mRNA expression of extracellular superoxide dismutase (EC-SOD) were investigated in 16 mouse tissues. We developed a double-antibody sandwich ELISA using the affinity-purified IgG against native mouse EC-SOD. EC-SOD could be detected in all of the tissues examined (lung, kidney, testis, brown fat, liver, adrenal gland, pancreas, colon, white fat, thymus, stomach, spleen, heart, skeletal muscle, ileum, and brain, in decreasing order of content measured as µg/g wet tissue). Lung showed a markedly higher value of EC-SOD than other tissues. Interestingly, white fat had a high content of EC-SOD in terms of micrograms per milligram protein, which corresponded to that of lung. Kidney showed the strongest expression of EC-SOD mRNA. Relatively strong expression of the mRNA was observed in lung, white fat, adrenal gland, brown fat, and testis. Heart and brain showed only weak signals, and no such expression could be detected in either digestive organs or skeletal muscle. Immunohistochemically, EC-SOD was localized mainly to connective tissues and vascular walls in the tissues examined. Deep staining in the cytosol was observed in the cortical tubular cells of kidney. These results suggest that EC-SOD is distributed systemically in mice and that the physiological importance of this enzyme may be a compensatory adaptation to oxidative stress, particularly in lung and kidney.

enzyme-linked immunosorbent assay; immunohistochemistry; lung; white fat; kidney


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