Am J Physiol Cell Physiol AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 275: C505-C514, 1998;
0363-6143/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gaur, S.
Right arrow Articles by Goodman, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gaur, S.
Right arrow Articles by Goodman, H. M.
Vol. 275, Issue 2, C505-C514, August 1998

Growth hormone regulates the distribution of L-type calcium channels in rat adipocyte membranes

Shikha Gaur1, Mary E. Morton2, G. Peter Frick1, and H. Maurice Goodman1

1 Department of Physiology, University of Massachusetts Medical School, Worcester 01655; and 2 Department of Biology, College of the Holy Cross, Worcester, Massachusetts 01610

Earlier studies demonstrated that deprivation of growth hormone (GH) for >= 3 h decreased basal and maximally stimulated cytosolic Ca2+ in rat adipocytes and suggested that membrane Ca2+ channels might be decreased. Measurement of L-type Ca2+ channels in purified plasma membranes by immunoassay or dihydropyridine binding indicated a two- to fourfold decrease after 3 h of incubation without GH. No such decrease was seen in unfractionated adipocyte membrane preparations. The decrease in plasma membrane channel content was largely accounted for by redistribution of channels to a light microsomal membrane fraction. Immunoassay of alpha 1-, alpha 2/delta -, and beta -channel subunits in membrane fractions indicated that the channels redistributed as intact complexes. Addition of GH during the 1st h of incubation prevented channel redistribution, and addition of GH after 3 h restored channel distribution to the GH-replete state of freshly isolated adipocytes. The studies suggest that GH may regulate the abundance of Ca2+ channels in the adipocyte plasma membrane and thereby modulate sensitivity to signals, the expression of which is Ca2+ dependent.

immunoassay; tritiated PN-200-110; cell fractionation; intracellular calcium concentration


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. G. Cammisotto and L. J. Bukowiecki
Role of calcium in the secretion of leptin from white adipocytes
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2004; 287(6): R1380 - R1386.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. R. Levy, J. Gyarmati, J. M. Lesko, R. A. Adler, and W. Stevens
Dual regulation of leptin secretion: intracellular energy and calcium dependence of regulated pathway
Am J Physiol Endocrinol Metab, May 1, 2000; 278(5): E892 - E901.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. H. Gravholt, O. Schmitz, L. Simonsen, J. Bulow, J. S. Christiansen, and N. Moller
Effects of a physiological GH pulse on interstitial glycerol in abdominal and femoral adipose tissue
Am J Physiol Endocrinol Metab, November 1, 1999; 277(5): E848 - E854.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online