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Am J Physiol Cell Physiol 275: C505-C514, 1998;
0363-6143/98 $5.00
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Vol. 275, Issue 2, C505-C514, August 1998

Growth hormone regulates the distribution of L-type calcium channels in rat adipocyte membranes

Shikha Gaur1, Mary E. Morton2, G. Peter Frick1, and H. Maurice Goodman1

1 Department of Physiology, University of Massachusetts Medical School, Worcester 01655; and 2 Department of Biology, College of the Holy Cross, Worcester, Massachusetts 01610

Earlier studies demonstrated that deprivation of growth hormone (GH) for >= 3 h decreased basal and maximally stimulated cytosolic Ca2+ in rat adipocytes and suggested that membrane Ca2+ channels might be decreased. Measurement of L-type Ca2+ channels in purified plasma membranes by immunoassay or dihydropyridine binding indicated a two- to fourfold decrease after 3 h of incubation without GH. No such decrease was seen in unfractionated adipocyte membrane preparations. The decrease in plasma membrane channel content was largely accounted for by redistribution of channels to a light microsomal membrane fraction. Immunoassay of alpha 1-, alpha 2/delta -, and beta -channel subunits in membrane fractions indicated that the channels redistributed as intact complexes. Addition of GH during the 1st h of incubation prevented channel redistribution, and addition of GH after 3 h restored channel distribution to the GH-replete state of freshly isolated adipocytes. The studies suggest that GH may regulate the abundance of Ca2+ channels in the adipocyte plasma membrane and thereby modulate sensitivity to signals, the expression of which is Ca2+ dependent.

immunoassay; tritiated PN-200-110; cell fractionation; intracellular calcium concentration


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