| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Laboratory of Cellular and Molecular Physiology, Departments of Anesthesiology and Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232; and 2 Division of Respiratory Diseases, Children's Hospital, Boston, Massachusetts 02115
Cell swelling activates an outwardly rectifying anion channel termed VSOAC (volume-sensitive organic osmolyte/anion channel). Regulation of VSOAC by intracellular electrolytes was characterized in Chinese hamster ovary cells by whole cell patch clamp. Elevation of intracellular CsCl concentration from 40 to 180 mM resulted in a concentration-dependent decrease in channel activation. Activation of VSOAC was insensitive to the salt gradient across the plasma membrane, the intracellular concentration of specific anions or cations, and the total intracellular concentration of cations, anions, or electrolytes. Comparison of cells dialyzed with either CsCl or Na2SO4 solutions demonstrated directly that VSOAC activation is modulated by intracellular ionic strength (µi). The relative cell volume at which VSOAC current activation was triggered, termed the channel volume set point, decreased with decreasing ionic strength. At µi = 0.04, VSOAC activation occurred spontaneously in shrunken cells. The rate of VSOAC activation was nearly 50-fold higher in cells with µi = 0.04 vs. those with µi = 0.18. We propose that µi modulates the volume sensor responsible for channel activation.
chloride channels; volume regulation; cell swelling; patch clamp; organic osmolytes
This article has been cited by other articles:
|
|
 |
|
  N. Okumura, S. Imai, F. Toyoda, E. Isoya, K. Kumagai, H. Matsuura, and Y. Matsusue Regulatory role of tyrosine phosphorylation in the swelling-activated chloride current in isolated rabbit articular chondrocytes J. Physiol., August 1, 2009; 587(15): 3761 - 3776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. K. Hoffmann, I. H. Lambert, and S. F. Pedersen Physiology of Cell Volume Regulation in Vertebrates Physiol Rev, January 1, 2009; 89(1): 193 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ben-Tabou De-Leon, G. Ben-Zeev, and I. Nussinovitch Effects of osmotic shrinkage on voltage-gated Ca2+ channel currents in rat anterior pituitary cells Am J Physiol Cell Physiol, January 1, 2006; 290(1): C222 - C232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Fischmeister and H. C. Hartzell Volume sensitivity of the bestrophin family of chloride channels J. Physiol., January 15, 2005; 562(2): 477 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nobles, C. F. Higgins, and A. Sardini Extracellular acidification elicits a chloride current that shares characteristics with ICl(swell) Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1426 - C1435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. B. Tomassen, D. Fekkes, H. R. de Jonge, and B. C. Tilly Osmotic swelling-provoked release of organic osmolytes in human intestinal epithelial cells Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1417 - C1422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lytle and T. McManus Coordinate modulation of Na-K-2Cl cotransport and K-Cl cotransport by cell volume and chloride Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1422 - C1431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Carton, D. Trouet, D. Hermans, H. Barth, K. Aktories, G. Droogmans, N. K. Jorgensen, E. K. Hoffmann, B. Nilius, and J. Eggermont RhoA exerts a permissive effect on volume-regulated anion channels in vascular endothelial cells Am J Physiol Cell Physiol, July 1, 2002; 283(1): C115 - C125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik Molecular Structure and Physiological Function of Chloride Channels Physiol Rev, April 1, 2002; 82(2): 503 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Y. Estevez, T. Bond, and K. Strange Regulation of ICl,swell in neuroblastoma cells by G protein signaling pathways Am J Physiol Cell Physiol, July 1, 2001; 281(1): C89 - C98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nehrke, T. Begenisich, J. Pilato, and J. E. Melvin Model Organisms: New Insights: Into Ion Channel and Transporter Function. Caenorhabditis elegans ClC-type chloride channels: novel variants and functional expression Am J Physiol Cell Physiol, December 1, 2000; 279(6): C2052 - C2066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Wittels, E. M. Hubert, M. W. Musch, and L. Goldstein Osmolyte channel regulation by ionic strength in skate RBC Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2000; 279(1): R69 - R76. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
