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Am J Physiol Cell Physiol 275: C382-C388, 1998;
0363-6143/98 $5.00
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Vol. 275, Issue 2, C382-C388, August 1998

Uptake and metabolism of biotin by human peripheral blood mononuclear cells

Janos Zempleni and Donald M. Mock

Department of Pediatrics (Gastroenterology), University of Arkansas for Medical Sciences and the Arkansas Children's Hospital Research Institute, Little Rock, Arkansas 72202

We studied the uptake of biotin into human peripheral blood mononuclear cells (PBMC) using [3H]biotin and studied the catabolism of biotin in PBMC using [14C]biotin. Over 30 min, [3H]biotin uptake was greater at 37°C than at 25°C (KT = 2.6 ± 0.4 nM, maximal velocity = 2.9 ± 0.2 fmol · 106 cells-1 · 30 min-1). Ouabain reduced [3H]biotin uptake to 65% of control values, suggesting that biotin uptake is Na-K-ATPase dependent. Unlabeled biotin and biotin analogs reduced the uptake of [3H]biotin to 22-70% of control values, suggesting the presence of a competition for a structurally specific biotin transporter. When endocytosis by PBMC was stimulated by various acyl glycerols, [3H]biotin uptake was 40-73% of control values; these data are consistent with the hypothesis that stimulated endocytosis reduces biotin transporter density on the cell surface. During a 168-h incubation, PBMC did not catabolize [14C]biotin.

1,2-dioctanoyl-sn-glycerol; endocytosis; sodium-potassium-adenosinetriphosphatase


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