| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Physiological Science, University of California, Los Angeles, Los Angeles, California 90095; and 2 Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235
The hypothesis that changes in muscle activation and loading regulate the expression and activity of neuronal nitric oxide (NO) synthase (nNOS) was tested using in vitro and in vivo approaches. Removal of weight bearing from rat hindlimb muscles for 10 days resulted in a significant decrease in nNOS protein and mRNA concentration in soleus muscles, which returned to control concentrations after return to weight bearing. Similarly, the concentration of nNOS in cultured myotubes increased by application of cyclic loading for 2 days. NO release from excised soleus muscles was increased significantly by a single passive stretch of 20% or by submaximal activation at 2 Hz, although the increases were not additive when both stimuli were applied simultaneously. Increased NO release resulting from passive stretch or activation was dependent on the presence of extracellular calcium. Cyclic loading of cultured myotubes also resulted in a significant increase in NO release. Together, these findings show that activity of muscle influences NO production in the short term, by regulating NOS activity, and in the long term, by regulating nNOS expression.
rat; calcium; contraction; muscle mechanics
This article has been cited by other articles:
|
|
 |
|
  M. J. Drummond, H. C. Dreyer, B. Pennings, C. S. Fry, S. Dhanani, E. L. Dillon, M. Sheffield-Moore, E. Volpi, and B. B. Rasmussen Skeletal muscle protein anabolic response to resistance exercise and essential amino acids is delayed with aging J Appl Physiol, May 1, 2008; 104(5): 1452 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Kosek and M. M. Bamman Modulation of the dystrophin-associated protein complex in response to resistance training in young and older men J Appl Physiol, May 1, 2008; 104(5): 1476 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Loyer, P. Oliviero, T. Damy, E. Robidel, F. Marotte, C. Heymes, and J.-L. Samuel Effects of sex differences on constitutive nitric oxide synthase expression and activity in response to pressure overload in rats Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2650 - H2658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Zhang, G. A. Truskey, and W. E. Kraus Effect of cyclic stretch on beta1D-integrin expression and activation of FAK and RhoA Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2057 - C2069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Pye, J. Palomero, T. Kabayo, and M. J. Jackson Real-time measurement of nitric oxide in single mature mouse skeletal muscle fibres during contractions J. Physiol., May 15, 2007; 581(1): 309 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Jackson, D. Pye, and J. Palomero The production of reactive oxygen and nitrogen species by skeletal muscle J Appl Physiol, April 1, 2007; 102(4): 1664 - 1670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Tidball and M. Wehling-Henricks The role of free radicals in the pathophysiology of muscular dystrophy J Appl Physiol, April 1, 2007; 102(4): 1677 - 1686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Vassilakopoulos and S. N. A. Hussain Ventilatory muscle activation and inflammation: cytokines, reactive oxygen species, and nitric oxide J Appl Physiol, April 1, 2007; 102(4): 1687 - 1695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Vassilakopoulos, K. Govindaraju, D. Parthenis, D. H. Eidelman, Y. Watanabe, and S. N. A. Hussain Nitric oxide production in the ventilatory muscles in response to acute resistive loading Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L1013 - L1022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Sellman, K. C. DeRuisseau, J. L. Betters, V. A. Lira, Q. A. Soltow, J. T. Selsby, and D. S. Criswell In vivo inhibition of nitric oxide synthase impairs upregulation of contractile protein mRNA in overloaded plantaris muscle J Appl Physiol, January 1, 2006; 100(1): 258 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Tidball Mechanical signal transduction in skeletal muscle growth and adaptation J Appl Physiol, May 1, 2005; 98(5): 1900 - 1908. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Tidball Inflammatory processes in muscle injury and repair Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2005; 288(2): R345 - R353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Zhang, W. E. Kraus, and G. A. Truskey Stretch-induced nitric oxide modulates mechanical properties of skeletal muscle cells Am J Physiol Cell Physiol, August 1, 2004; 287(2): C292 - C299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Clifford and Y. Hellsten Vasodilatory mechanisms in contracting skeletal muscle J Appl Physiol, July 1, 2004; 97(1): 393 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fluck, M. Chiquet, S. Schmutz, M.-H. Mayet-Sornay, and D. Desplanches Reloading of atrophied rat soleus muscle induces tenascin-C expression around damaged muscle fibers Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2003; 284(3): R792 - R801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kumar, I. Chaudhry, M. B. Reid, and A. M. Boriek Distinct Signaling Pathways Are Activated in Response to Mechanical Stress Applied Axially and Transversely to Skeletal Muscle Fibers J. Biol. Chem., November 22, 2002; 277(48): 46493 - 46503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Powell, B. L. Smiley, J. Mills, and H. H. Vandenburgh Mechanical stimulation improves tissue-engineered human skeletal muscle Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1557 - C1565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Tatsumi, A. Hattori, Y. Ikeuchi, J. E. Anderson, and R. E. Allen Release of Hepatocyte Growth Factor from Mechanically Stretched Skeletal Muscle Satellite Cells and Role of pH and Nitric Oxide Mol. Biol. Cell, August 1, 2002; 13(8): 2909 - 2918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wehling, M. J. Spencer, and J. G. Tidball A nitric oxide synthase transgene ameliorates muscular dystrophy in mdx mice J. Cell Biol., October 1, 2001; 155(1): 123 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Stamler and G. Meissner Physiology of Nitric Oxide in Skeletal Muscle Physiol Rev, January 1, 2001; 81(1): 209 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Frandsen, L. Hoffner, A. Betak, B. Saltin, J. Bangsbo, and Y. Hellsten Endurance training does not alter the level of neuronal nitric oxide synthase in human skeletal muscle J Appl Physiol, September 1, 2000; 89(3): 1033 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Koh and J. G. Tidball Nitric oxide inhibits calpain-mediated proteolysis of talin in skeletal muscle cells Am J Physiol Cell Physiol, September 1, 2000; 279(3): C806 - C812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Tidball, M. J. Spencer, M. Wehling, and E. Lavergne Nitric-oxide Synthase Is a Mechanical Signal Transducer That Modulates Talin and Vinculin Expression J. Biol. Chem., November 12, 1999; 274(46): 33155 - 33160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Roberts, R. J. Barnard, A. Jasman, and T. W. Balon Acute exercise increases nitric oxide synthase activity in skeletal muscle Am J Physiol Endocrinol Metab, August 1, 1999; 277(2): E390 - E394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Bredt NO skeletal muscle derived relaxing factor in Duchenne muscular dystrophy PNAS, December 8, 1998; 95(25): 14592 - 14593. [Full Text] [PDF]
|
|
|
|
|
|
L. W. Smith, J. D. Smith, and D. S. Criswell Involvement of nitric oxide synthase in skeletal muscle adaptation to chronic overload J Appl Physiol, May 1, 2002; 92(5): 2005 - 2011. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
