Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 275: C208-C215, 1998;
0363-6143/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Faure, V.
Right arrow Articles by Goureau, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Faure, V.
Right arrow Articles by Goureau, O.
Vol. 275, Issue 1, C208-C215, July 1998

Tyrosine kinase inhibitors and antioxidants modulate NF-kappa B and NOS-II induction in retinal epithelial cells

Violaine Faure, Yves Courtois, and Olivier Goureau

Développement, Vieillissement, et Pathologie de la Rétine, Unité 450 Institut National de la Santé et de la Recherche Médicale, Association Claude Bernard, 75016 Paris, France

Bovine retinal pigmented epithelial (RPE) cells express an inducible nitric oxide synthase (NOS-II) after activation with interferon-gamma (IFN-gamma ) and lipopolysaccharide (LPS). Experiments were performed to investigate the effects of tyrosine kinase inhibitors (genistein and herbimycin A) and antioxidants [pyrrolidine dithiocarbamate (PDTC) and butyl hydroxyanisol] on NOS-II induction. The LPS-IFN-gamma -induced nitrite release was inhibited in a concentration-dependent manner by these compounds. Analysis by Northern blot showed that this inhibitory effect correlated with a decrease in NOS-II mRNA accumulation. Analysis by electrophoretic mobility shift assay of the activation of the transcription factor nuclear factor-kappa B (NF-kappa B) involved in NOS-II induction demonstrated that LPS alone or combined with IFN-gamma induced NF-kappa B binding. NF-kappa B activation was not changed by the presence of tyrosine kinase inhibitors but was totally prevented by PDTC pretreatment. Immunocytochemistry experiments confirmed the reduction of the nuclear translocation of NF-kappa B only by PDTC. Our results demonstrated the existence in retinal pigmented epithelial cells of different intracellular signaling pathways in NOS-II induction, since tyrosine kinase inhibitors blocked NOS-II mRNA accumulation without inhibiting NF-kappa B activation. Furthermore, the LPS-IFN-gamma -induced NOS-II mRNA accumulation was sensitive to cycloheximide, suggesting that, in addition to NF-kappa B, transcriptional factors that require new protein synthesis are involved in NOS-II induction.

interferon-gamma ; lipopolysaccharide; nitric oxide; transcription factor


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
M. Koyama, S. S. Spicer, and B. A. Schulte
Distribution of I{kappa}B Proteins in Gastric Mucosa and Other Organs of Mouse and Gerbil
J. Histochem. Cytochem., February 1, 2000; 48(2): 191 - 200.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. M. Badger, M. N. Cook, B. A. Swift, T. M. Newman-Tarr, M. Gowen, and M. Lark
Inhibition of Interleukin-1-Induced Proteoglycan Degradation and Nitric Oxide Production in Bovine Articular Cartilage/Chondrocyte Cultures by the Natural Product, Hymenialdisine
J. Pharmacol. Exp. Ther., August 1, 1999; 290(2): 587 - 593.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. Colasanti, T. Persichini, E. Cavalieri, C. Fabrizi, S. Mariotto, M. Menegazzi, G. M. Lauro, and H. Suzuki
Rapid Inactivation of NOS-I by Lipopolysaccharide Plus Interferon-gamma -induced Tyrosine Phosphorylation
J. Biol. Chem., April 9, 1999; 274(15): 9915 - 9917.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Faure, C. Hecquet, Y. Courtois, and O. Goureau
Role of Interferon Regulatory Factor-1 and Mitogen-activated Protein Kinase Pathways in the Induction of Nitric Oxide Synthase-2 in Retinal Pigmented Epithelial Cells
J. Biol. Chem., February 19, 1999; 274(8): 4794 - 4800.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online