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Department of Pediatrics, Louisiana State University Medical Center in New Orleans, New Orleans, Louisiana 70112
To assess the
role of distal nephron apical Na channel (ENaC) gene expression in Na
wasting by the immature kidney, ENaC 
-, 
-, and 
-subunit mRNA
levels were examined in the rat by RT-PCR. In microdissected nephron
segments, all three ENaC subunit mRNAs were detected in the distal
convoluted tubule, connecting tubule, cortical collecting duct, and
outer medullary collecting duct. The inner medullary collecting duct
and all other nephron segments were consistently negative. The mRNA
levels were quantified in kidneys at different developmental stages by
multiplex RT-PCR with "primer dropping," with endoplasmic
reticulum-specific cyclophilin mRNA as an internal standard. All three
ENaC mRNA levels were low or undetectable on gestational
day 16 and only slightly higher 3 days
before birth. A sharp rise was observed between 3 days before and
1-3 days after birth; the levels at postnatal
days 1-3 were already similar to
those of adult kidneys. The results suggest that ENaC subunit gene
expression is not a limiting factor in the full-term newborn rat
kidney, but low levels of expression may limit distal Na absorption in
more immature kidneys, such as those of very premature human infants.
sodium transport; collecting duct; renal ontogeny; multiplex reverse transcription-polymerase chain reaction; capillary electrophoresis with laser-induced fluorescent detection
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