Am J Physiol Cell Physiol Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 274: C1601-C1607, 1998;
0363-6143/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Youn, T.
Right arrow Articles by Hai, C.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Youn, T.
Right arrow Articles by Hai, C.-M.
Vol. 274, Issue 6, C1601-C1607, June 1998

Length-dependent modulation of smooth muscle activation: effects of agonist, cytochalasin, and temperature

Thomas Youn, Son A. Kim, and Chi-Ming Hai

Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, Rhode Island 02912

We tested the hypothesis that mechanical strain modulates agonist sensitivity of smooth muscle by measuring myosin phosphorylation and contractile force in bovine tracheal smooth muscle activated by various concentrations of the muscarinic receptor agonist carbachol and at various muscle lengths. Increasing carbachol concentration by 10,000-fold did not restore myosin phosphorylation levels at shorter muscle lengths to the level at optimal length (Lo). Maximum levels of myosin phosphorylation induced by carbachol at 0.6, 0.8, and 1.0 Lo were similar but became lower at <0.6 Lo. Cytochalasin D significantly attenuated carbachol-induced contraction by 54%. In addition, cytochalasin D treatment induced a parallel downward shift in the length-myosin phosphorylation relation. Lowering temperature from 37 to 23°C did not significantly change the length dependencies of carbachol-induced active force and myosin phosphorylation. These results have led us to conclude that 1) agonist sensitivity and maximum level of activation (as measured by myosin phosphorylation) are targets of length-dependent modulation, 2) actin filaments involved in contraction and length-dependent modulation are distinct in sensitivity to cytochalasin D, and 3) length-dependent modulation is relatively temperature insensitive.

acetylcholine; actin; myogenic response; myosin phosphorylation; stretch


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
S. J. Gunst and W. Zhang
Actin cytoskeletal dynamics in smooth muscle: a new paradigm for the regulation of smooth muscle contraction
Am J Physiol Cell Physiol, September 1, 2008; 295(3): C576 - C587.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. Chen, G. Liu, F. Shardonofsky, M. Dowell, O. Lakser, R. W. Mitchell, J. J. Fredberg, L. H. Pinto, and J. Solway
Tidal breathing pattern differentially antagonizes bronchoconstriction in C57BL/6J vs. A/J mice
J Appl Physiol, July 1, 2006; 101(1): 249 - 255.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W. Zhang, Y. Wu, L. Du, D. D. Tang, and S. J. Gunst
Activation of the Arp2/3 complex by N-WASp is required for actin polymerization and contraction in smooth muscle
Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1145 - C1160.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. R. Bai, J. H. T. Bates, V. Brusasco, B. Camoretti-Mercado, P. Chitano, L. H. Deng, M. Dowell, B. Fabry, L. E. Ford, J. J. Fredberg, et al.
On the terminology for describing the length-force relationship and its changes in airway smooth muscle
J Appl Physiol, December 1, 2004; 97(6): 2029 - 2034.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. D. Tang and J. Tan
Downregulation of profilin with antisense oligodeoxynucleotides inhibits force development during stimulation of smooth muscle
Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1528 - H1536.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C.-M. Hai, G. Sadowska, L. Francois, and B. S. Stonestreet
Maternal dexamethasone treatment alters myosin isoform expression and contractile dynamics in fetal arteries
Am J Physiol Heart Circ Physiol, November 1, 2002; 283(5): H1743 - H1749.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
W. T. Gerthoffer and S. J. Gunst
Signal Transduction in Smooth Muscle: Invited Review: Focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle
J Appl Physiol, August 1, 2001; 91(2): 963 - 972.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. Y. Seow
Response of arterial smooth muscle to length perturbation
J Appl Physiol, November 1, 2000; 89(5): 2065 - 2072.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. S. An and C.-M. Hai
Mechanical signals and mechanosensitive modulation of intracellular [Ca2+] in smooth muscle
Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1375 - C1384.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W.-L. Chan, J. Silberstein, and C.-M. Hai
Mechanical strain memory in airway smooth muscle
Am J Physiol Cell Physiol, May 1, 2000; 278(5): C895 - C904.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. S. An and C.-M. Hai
Mechanical strain modulates maximal phosphatidylinositol turnover in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, November 1, 1999; 277(5): L968 - L974.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online