Am J Physiol Cell Physiol AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 274: C1424-C1428, 1998;
0363-6143/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gudi, S. R. P.
Right arrow Articles by Frangos, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gudi, S. R. P.
Right arrow Articles by Frangos, J. A.
Vol. 274, Issue 5, C1424-C1428, May 1998

RAPID COMMUNICATION
Equibiaxial strain and strain rate stimulate early activation of G proteins in cardiac fibroblasts

Siva R. P. Gudi, Ann A. Lee, Craig B. Clark, and John A. Frangos

Department of Bioengineering, University of California, San Diego, La Jolla, California 92093

Cardiac fibroblasts are responsible for the production of the extracellular matrix of the heart, with alterations of fibroblast function implicated in myocardial infarction and cardiac hypertrophy. Here the role of heterotrimeric GTP-binding proteins (G proteins) in the mechanotransduction of strain in rat cardiac fibroblasts was investigated. Cells in an equibiaxial stretch device were incubated with the photoreactive GTP analog azidoanalido [alpha -32P]GTP (AAGTP) and were subjected to various regimens of strain. Autoradiographic analysis showed a 42-kDa protein labeled for cells exposed to 12 cycles of 3% strain or 6 cycles of 6% strain over 60 s (strain rate of 1.2%/s), whereas 6 cycles of 3% strain (0.6%/s) elicited no measurable response. To further investigate the role of strain rate, a single 6% cycle over 10 or 60 s (1.2% and 0.2%/s, respectively) was applied, with the more rapid cycle stimulating AAGTP binding, whereas the lower strain rate showed no response. In cells subjected to a single 6% cycle/10 s, immunoprecipitation identified the AAGTP-labeled 42-kDa band as the G protein subunits Galpha q and Galpha i1. These results demonstrate that G protein activation represents one of the early mechanotransduction events in cardiac fibroblasts subjected to mechanical strain, with the rate at which the strain is applied modulating this response.

cell mechanics; mechanical stretch; mechanotransduction


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Wang, B. S. Maciejewski, N. Lee, O. Silbert, N. L. McKnight, J. A. Frangos, and J. Sanchez-Esteban
Strain-induced fetal type II epithelial cell differentiation is mediated via cAMP-PKA-dependent signaling pathway
Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L820 - L827.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. A. Hornberger, D. D. Armstrong, T. J. Koh, T. J. Burkholder, and K. A. Esser
Intracellular signaling specificity in response to uniaxial vs. multiaxial stretch: implications for mechanotransduction
Am J Physiol Cell Physiol, January 1, 2005; 288(1): C185 - C194.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. J. Olearczyk, A. H. Stephenson, A. J. Lonigro, and R. S. Sprague
Heterotrimeric G protein Gi is involved in a signal transduction pathway for ATP release from erythrocytes
Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H940 - H945.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. S. Chockalingam, R. Cholera, S. A. Oak, Y. Zheng, H. W. Jarrett, and D. B. Thomason
Dystrophin-glycoprotein complex and Ras and Rho GTPase signaling are altered in muscle atrophy
Am J Physiol Cell Physiol, August 1, 2002; 283(2): C500 - C511.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. Correa-Meyer, L. Pesce, C. Guerrero, and J. I. Sznajder
Mechanotransduction in the Lung: Cyclic stretch activates ERK1/2 via G proteins and EGFR in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L883 - L891.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Shafrir and G. Forgacs
Mechanotransduction through the cytoskeleton
Am J Physiol Cell Physiol, March 1, 2002; 282(3): C479 - C486.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. P. Provenzano, D. Heisey, K. Hayashi, R. Lakes, and R. Vanderby Jr.
Subfailure damage in ligament: a structural and cellular evaluation
J Appl Physiol, January 1, 2002; 92(1): 362 - 371.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
C. R. White, M. Haidekker, X. Bao, and J. A. Frangos
Temporal Gradients in Shear, but Not Spatial Gradients, Stimulate Endothelial Cell Proliferation
Circulation, May 22, 2001; 103(20): 2508 - 2513.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. A. Hill, H. Zou, M. J. Davis, S. J. Potocnik, and S. Price
Transient increases in diameter and [Ca2+]i are not obligatory for myogenic constriction
Am J Physiol Heart Circ Physiol, February 1, 2000; 278(2): H345 - H352.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. J. Davis and M. A. Hill
Signaling Mechanisms Underlying the Vascular Myogenic Response
Physiol Rev, April 1, 1999; 79(2): 387 - 423.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S.-Y. Shai, A. E. Harpf, C. J. Babbitt, M. C. Jordan, M. C. Fishbein, J. Chen, M. Omura, T. A. Leil, K. D. Becker, M. Jiang, et al.
Cardiac Myocyte-Specific Excision of the {beta}1 Integrin Gene Results in Myocardial Fibrosis and Cardiac Failure
Circ. Res., March 8, 2002; 90(4): 458 - 464.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online