Am J Physiol Cell Physiol AJP: Gastrointestinal and Liver Physiology
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Am J Physiol Cell Physiol 274: C1332-C1345, 1998;
0363-6143/98 $5.00
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Vol. 274, Issue 5, C1332-C1345, May 1998

Aquaporins in complex tissues: distribution of aquaporins 1-5 in human and rat eye

Steffen Hamann1, Thomas Zeuthen1, Morten La Cour2, Erlend A. Nagelhus3, Ole Petter Ottersen3, Peter Agre4, and Søren Nielsen5

1 Department of Medical Physiology, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen; 2 Eye Department and Eye Pathology Institute, Rigshospitalet, DK-2200 Copenhagen; 5 Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus, Denmark; 3 Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway; and 4 Departments of Biological Chemistry and Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland 21205

Multiple physiological fluid movements are involved in vision. Here we define the cellular and subcellular sites of aquaporin (AQP) water transport proteins in human and rat eyes by immunoblotting, high-resolution immunocytochemistry, and immunoelectron microscopy. AQP3 is abundant in bulbar conjunctival epithelium and glands but is only weakly present in corneal epithelium. In contrast, AQP5 is prominent in corneal epithelium and apical membranes of lacrimal acini. AQP1 is heavily expressed in scleral fibroblasts, corneal endothelium and keratocytes, and endothelium covering the trabecular meshwork and Schlemm's canal. Although AQP1 is plentiful in ciliary nonpigmented epithelium, it is not present in ciliary pigmented epithelium. Posterior and anterior epithelium of the iris and anterior lens epithelium also contain significant amounts of AQP1, but AQP0 (major intrinsic protein of the lens) is expressed in lens fiber cells. Retinal Müller cells and astrocytes exhibit notable concentrations of AQP4, whereas neurons and retinal pigment epithelium do not display aquaporin immunolabeling. These studies demonstrate selective expression of AQP1, AQP3, AQP4, and AQP5 in distinct ocular epithelia, predicting specific roles for each in the complex network through which water movements occur in the eye.

water transporters; cornea; uvea; lens; retina


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