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Am J Physiol Cell Physiol 274: C1324-C1331, 1998;
0363-6143/98 $5.00
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Vol. 274, Issue 5, C1324-C1331, May 1998

Modulation of human neuronal alpha 1E-type calcium channel by alpha 2delta -subunit

Ning Qin1, Riccardo Olcese1, Enrico Stefani1,2, and Lutz Birnbaumer1,3

Departments of 1 Anesthesiology, 2 Physiology, and 3 Biological Chemistry and Molecular Biology Institute, School of Medicine, University of California, Los Angeles, California 90095

Calcium channels are composed of a pore-forming subunit, alpha 1, and at least two auxiliary subunits, beta - and alpha 2delta -subunits. It is well known that beta -subunits regulate most of the properties of the channel. The function of alpha 2delta -subunit is less understood. In this study, the effects of the calcium channel alpha 2delta -subunit on the neuronal alpha 1E voltage-gated calcium channel expressed in Xenopus oocytes was investigated without and with simultaneous coexpression of either the beta 1b- or the beta 2a-subunit. Most aspects of alpha 1E function were affected by alpha 2delta . Thus alpha 2delta caused a shift in the current-voltage and conductance-voltage curves toward more positive potentials and accelerated activation, deactivation, and the installation of the inactivation process. In addition, the efficiency with which charge movement is coupled to pore opening assessed by determining ratios of limiting conductance to limiting charge movement was decreased by alpha 2delta by factors that ranged from 1.6 (P < 0.01) for alpha 1E-channels to 3.0 (P < 0.005) for alpha 1Ebeta 1b-channels. These results indicate that alpha 2delta facilitates the expression and the maturation of alpha 1E-channels and converts these channels into molecules responding more rapidly to voltage.

calcium channel alpha 2delta -subunit; coupling efficiency; facilitation of expression


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