| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Gastrointestinal Division, Departments of Medicine and Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2195
Intestinal neutral NaCl absorption, which is made up of
brush-border (BB)
Na+/H+
exchange linked to BB
Cl
/HCO3
exchange, is up- and downregulated as part of digestion and diarrheal
diseases. Glucocorticoids stimulate ileal NaCl absorption and BB
Na+/H+
exchange. Intestinal BB contains two
Na+/H+
exchanger isoforms, NHE2 and NHE3, but their relative roles in rabbit
ileal BB
Na+/H+
exchange has not been determined. A technique to separate the contribution of NHE2 and NHE3 to ileal BB
Na+/H+
exchange activity was standardized by using an amiloride-related compound, HOE-694. Under basal conditions, both NHE2 and NHE3 contribute ~50% to ileal
Na+/H+
exchange. Glucocorticoids (methylprednisolone) increase BB
Na+/H+
exchange (2.5 times) but increase only ileal NHE3 activity (4.1 times),
without an effect on NHE2 activity. Thus ileal BB
Na+/H+
exchange in animals treated with glucocorticoids is 69% via NHE3. A
quantitative Western analysis for NHE3 was developed, using as an
internal standard a fusion protein of the COOH-terminal 85 amino acids
of NHE3 and maltose binding protein. Glucocorticoid treatment increased
the amount of BB NHE3. The quantitative Western analysis showed that
NHE3 makes up 0.018% of ileal BB protein in control rabbits and
0.042% (2.3 times as much) in methylprednisolone-treated rabbits.
Methylprednisolone treatment did not alter the amount of ileal BB NHE2
protein. NHE3 turnover number was estimated to be 458 cycles/s under
basal conditions and 708 cycles/s in glucocorticoid-treated ileum. Thus
methylprednisolone stimulates ileal BB
Na+/H+
exchange activity only by an effect on NHE3 and not on NHE2; it does so
primarily by increasing the amount of BB NHE3, although it also
increases the NHE3 turnover number.
HOE-694; plasma membrane vesicle; quantitative Western analysis; turnover number
This article has been cited by other articles:
|
|
 |
|
  S. B. Subramanya, V. M. Rajendran, P. Srinivasan, N. S. Nanda Kumar, B. S. Ramakrishna, and H. J. Binder Differential regulation of cholera toxin-inhibited Na-H exchange isoforms by butyrate in rat ileum Am J Physiol Gastrointest Liver Physiol, October 1, 2007; 293(4): G857 - G863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Donowitz and X. Li Regulatory Binding Partners and Complexes of NHE3 Physiol Rev, July 1, 2007; 87(3): 825 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Moeser, P. K. Nighot, K. A. Ryan, J. G. Wooten, and A. T. Blikslager Prostaglandin-mediated inhibition of Na+/H+ exchanger isoform 2 stimulates recovery of barrier function in ischemia-injured intestine Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G885 - G894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sandu, F. Artunc, M. Palmada, R. Rexhepaj, F. Grahammer, A. Hussain, C. Yun, D. R. Alessi, and F. Lang Impaired intestinal NHE3 activity in the PDK1 hypomorphic mouse Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G868 - G876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Grahammer, G. Henke, C. Sandu, R. Rexhepaj, A. Hussain, B. Friedrich, T. Risler, M. Metzger, L. Just, T. Skutella, et al. Intestinal function of gene-targeted mice lacking serum- and glucocorticoid-inducible kinase 1 Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1114 - G1123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Bachmann, B. Riederer, H. Rossmann, S. Groos, P. J. Schultheis, G. E. Shull, M. Gregor, M. P. Manns, and U. Seidler The Na+/H+ exchanger isoform 2 is the predominant NHE isoform in murine colonic crypts and its lack causes NHE3 upregulation Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G125 - G133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Musch, C. Bookstein, F. Rocha, A. Lucioni, H. Ren, J. Daniel, Y. Xie, R. L. McSwine, M. C. Rao, J. Alverdy, et al. Region-specific adaptation of apical Na/H exchangers after extensive proximal small bowel resection Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G975 - G985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. R. Gawenis, X. Stien, G. E. Shull, P. J. Schultheis, A. L. Woo, N. M. Walker, and L. L. Clarke Intestinal NaCl transport in NHE2 and NHE3 knockout mice Am J Physiol Gastrointest Liver Physiol, May 1, 2002; 282(5): G776 - G784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Yun, Y. Chen, and F. Lang Glucocorticoid Activation of Na+/H+ Exchanger Isoform 3 Revisited. THE ROLES OF SGK1 AND NHERF2 J. Biol. Chem., March 1, 2002; 277(10): 7676 - 7683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ledoussal, A. L. Woo, M. L. Miller, and G. E. Shull Loss of the NHE2 Na+/H+ exchanger has no apparent effect on diarrheal state of NHE3-deficient mice Am J Physiol Gastrointest Liver Physiol, December 1, 2001; 281(6): G1385 - G1396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Cavet, S. Akhter, R. Murtazina, F. Sanchez de Medina, C.-M. Tse, and M. Donowitz Half-lives of plasma membrane Na+/H+ exchangers NHE1-3: plasma membrane NHE2 has a rapid rate of degradation Am J Physiol Cell Physiol, December 1, 2001; 281(6): C2039 - C2048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Turner and E. D. Black NHE3-dependent cytoplasmic alkalinization is triggered by Na+-glucose cotransport in intestinal epithelia Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1533 - C1541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Chambrey, P. L. St. John, D. Eladari, F. Quentin, D. G. Warnock, D. R. Abrahamson, R.-A. Podevin, and M. Paillard Localization and functional characterization of Na+/H+ exchanger isoform NHE4 in rat thick ascending limbs Am J Physiol Renal Physiol, October 1, 2001; 281(4): F707 - F717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Repishti, D. L. Hogan, V. Pratha, L. Davydova, M. Donowitz, C. M. Tse, and J. I. Isenberg Human duodenal mucosal brush border Na+/H+ exchangers NHE2 and NHE3 alter net bicarbonate movement Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G159 - G163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mac Laughlin, M. C. Damasco, P. Igarreta, and C. Amorena In vitro and in vivo evaluation of proximal tubular acidification in aging rats Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2001; 280(6): R1627 - R1631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Rocha, M. W. Musch, L. Lishanskiy, C. Bookstein, K. Sugi, Y. Xie, and E. B. Chang IFN-{gamma} downregulates expression of Na+/H+ exchangers NHE2 and NHE3 in rat intestine and human Caco-2/bbe cells Am J Physiol Cell Physiol, May 1, 2001; 280(5): C1224 - C1232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Rajendran, J. Geibel, and H. J. Binder Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon Am J Physiol Gastrointest Liver Physiol, March 1, 2001; 280(3): G400 - G405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Kiela, Y. S. Guner, H. Xu, J. F. Collins, and F. K. Ghishan Age- and tissue-specific induction of NHE3 by glucocorticoids in the rat small intestine Am J Physiol Cell Physiol, April 1, 2000; 278(4): C629 - C637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-W. Chow, S. Khurana, M. Woodside, S. Grinstein, and J. Orlowski The Epithelial Na+/H+ Exchanger, NHE3, Is Internalized through a Clathrin-mediated Pathway J. Biol. Chem., December 31, 1999; 274(53): 37551 - 37558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Cavet, S. Akhter, F. S. de Medina, M. Donowitz, and C.-M. Tse Na+/H+ exchangers (NHE1-3) have similar turnover numbers but different percentages on the cell surface Am J Physiol Cell Physiol, December 1, 1999; 277(6): C1111 - C1121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Alper, H. Rossmann, S. Wilhelm, A. K. Stuart-Tilley, B. E. Shmukler, and U. Seidler Expression of AE2 anion exchanger in mouse intestine Am J Physiol Gastrointest Liver Physiol, August 1, 1999; 277(2): G321 - G332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-W. Chow, M. Woodside, N. Demaurex, F. H. Yu, P. Plant, D. Rotin, S. Grinstein, and J. Orlowski Proline-rich Motifs of the Na+/H+ Exchanger 2 Isoform. BINDING OF Src HOMOLOGY DOMAIN 3 AND ROLE IN APICAL TARGETING IN EPITHELIA J. Biol. Chem., April 9, 1999; 274(15): 10481 - 10488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ikuma, M. Kashgarian, H. J. Binder, and V. M. Rajendran Differential regulation of NHE isoforms by sodium depletion in proximal and distal segments of rat colon Am J Physiol Gastrointest Liver Physiol, February 1, 1999; 276(2): G539 - G549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Donowitz, S. Khurana, C. M. Tse, and C. H. C. Yun III. Asymmetry in plasma membrane signal transduction: lessons from brush-border Na+/H+ exchangers Am J Physiol Gastrointest Liver Physiol, June 1, 1998; 274(6): G971 - G977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Donowitz, C. De La Horra, M. L. Calonge, I. S. Wood, J. Dyer, S. M. Gribble, F. S. De Medina, C. M. Tse, S. P. Shirazi-Beechey, and A. A. Ilundain In birds, NHE2 is major brush-border Na+/H+ exchanger in colon and is increased by a low-NaCl diet Am J Physiol Regulatory Integrative Comp Physiol, June 1, 1998; 274(6): R1659 - R1669. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
