Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 274: C1253-C1260, 1998;
0363-6143/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bremerich, D. H.
Right arrow Articles by Jones, K. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bremerich, D. H.
Right arrow Articles by Jones, K. A.
Vol. 274, Issue 5, C1253-C1260, May 1998

Effect of phorbol esters on Ca2+ sensitivity and myosin light-chain phosphorylation in airway smooth muscle

Dorothee H. Bremerich, Tetsuya Kai, David O. Warner, and Keith A. Jones

Departments of Anesthesiology and Physiology and Biophysics, Mayo Clinic and Foundation, Rochester, Minnesota 55905

We studied in beta -escin-permeabilized canine tracheal smooth muscle (CTSM) the effect of the protein kinase C (PKC) agonist phorbol 12,13-dibutyrate (PDBu) on isometric force at a constant submaximal Ca2+ concentration (i.e., the effect on Ca2+ sensitivity) and regulatory myosin light-chain (rMLC) phosphorylation. PDBu increased Ca2+ sensitivity, an increase associated with a concentration-dependent, sustained increase in rMLC phosphorylation. PDBu altered the relationship between rMLC phosphorylation and isometric force such that the increase in isometric force was less than that expected for the increase in rMLC phosphorylation observed. The effect of four PKC inhibitors [calphostin C, chelerythrine chloride, a pseudosubstrate inhibitor for PKC, PKC peptide-(19---31) (PSSI), and staurosporine] on PDBu-induced Ca2+ sensitization as well as the effect of calphostin C and PSSI on rMLC phosphorylation were determined. Whereas none of these compounds prevented or reversed the PDBu-induced increase in Ca2+ sensitivity, the PDBu-induced increase in rMLC phosphorylation was inhibited. We conclude that PDBu increases rMLC phosphorylation by activation of PKC but that the associated PDBu-induced increases in Ca2+ sensitivity are mediated by mechanisms other than activation of PKC in permeabilized airway smooth muscle.

beta -escin; lung; trachea; canine; protein kinase C inhibitors; activator; phorbol 12,13-dibutyrate; second messenger systems; protein kinase C


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Bai and M. J. Sanderson
Modulation of the Ca2+ sensitivity of airway smooth muscle cells in murine lung slices
Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L208 - L221.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C.-M. Hai and H. R. Kim
An expanded latch-bridge model of protein kinase C-mediated smooth muscle contraction
J Appl Physiol, April 1, 2005; 98(4): 1356 - 1365.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. J. Perkins, R. R. Lorenz, M. Bogoger, D. O. Warner, C. R. Cremo, and K. A. Jones
A novel mechanism by which hydrogen peroxide decreases calcium sensitivity in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L324 - L332.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. J. Janssen
Ionic mechanisms and Ca2+ regulation in airway smooth muscle contraction: do the data contradict dogma?
Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1161 - L1178.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Yoshimura, K. A. Jones, W. J. Perkins, T. Kai, and D. O. Warner
Calcium sensitization produced by G protein activation in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L631 - L638.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
M. Hanazaki, K. A. Jones, W. J. Perkins, and D. O. Warner
The Effects of Ethanol on Ca2+ Sensitivity in Airway Smooth Muscle
Anesth. Analg., March 1, 2001; 92(3): 767 - 774.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Kai, H. Yoshimura, K. A. Jones, and D. O. Warner
Relationship between force and regulatory myosin light chain phosphorylation in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L52 - L58.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online