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Am J Physiol Cell Physiol 274: C947-C957, 1998;
0363-6143/98 $5.00
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Vol. 274, Issue 4, C947-C957, April 1998

Time-dependent stimulation by aldosterone of blocker-sensitive ENaCs in A6 epithelia

Sandy I. Helman1, Xuehong Liu1, Kieron Baldwin3, Bonnie L. Blazer-Yost2, and Willem J. Els3

1 Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801; 2 Department of Biology, Indiana University-Purdue University at Indianapolis and Veterans Affairs Medical Center, Indianapolis, Indiana 46202; and 3 Department of Anatomy and Cell Biology, University of Cape Town Medical School, Cape Town, South Africa

To study and define the early time-dependent response (<= 6 h) of blocker-sensitive epithelial Na+ channels (ENaCs) to stimulation of Na+ transport by aldosterone, we used a new modified method of blocker-induced noise analysis to determine the changes of single-channel current (iNa) channel open probability (Po), and channel density (NT) under transient conditions of transport as measured by macroscopic short-circuit currents (Isc). In three groups of experiments in which spontaneous baseline rates of transport averaged 1.06, 5.40, and 15.14 µA/cm2, stimulation of transport occurred due to increase of blocker-sensitive channels. NT varied linearly over a 70-fold range of transport (0.5-35 µA/cm2). Relatively small and slow time-dependent but aldosterone-independent decreases of Po occurred during control (10-20% over 2 h) and aldosterone experimental periods (10-30% over 6 h). When the Po of control and aldosterone-treated tissues was examined over the 70-fold extended range of Na+ transport, Po was observed to vary inversely with Isc, falling from ~0.5 to ~0.15 at the highest rates of Na+ transport or ~25% per 3-fold increase of transport. Because decreases of Po from any source cannot explain stimulation of transport by aldosterone, it is concluded that the early time-dependent stimulation of Na+ transport in A6 epithelia is due exclusively to increase of apical membrane NT.

electrophysiology; epithelial sodium channels; tissue culture; cortical collecting ducts; kidney; noise analysis; amiloride


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