Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 274: C430-C439, 1998;
0363-6143/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pérez, C. F.
Right arrow Articles by Hidalgo, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pérez, C. F.
Right arrow Articles by Hidalgo, C.
Vol. 274, Issue 2, C430-C439, February 1998

Cyclic ADP-ribose activates caffeine-sensitive calcium channels from sea urchin egg microsomes

Claudio F. Pérez1, Juan José Marengo1, Ricardo Bull1, and Cecilia Hidalgo1,2

1 Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, and 2 Centro de Estudios Científicos de Santiago, Santiago 9, Chile

Adenosine 5'-cyclic diphosphoribose [cyclic ADP-ribose (cADPR)], a metabolite of NAD+ that promotes Ca2+ release from sea urchin egg homogenates and microsomal fractions, has been proposed to act as an endogenous agonist of Ca2+ release in sea urchin eggs. We describe experiments showing that a microsomal fraction isolated from Tetrapigus nyger sea urchin eggs displayed Ca2+-selective single channels with conductances of 155.0 ± 8.0 pS in asymmetric Cs+ solutions and 47.5 ± 1.1 pS in asymmetric Ca2+ solutions. These channels were sensitive to stimulation by Ca2+, ATP, and caffeine, but not inositol 1,4,5-trisphosphate, and were inhibited by ruthenium red. The channels were also activated by cADP-ribose in a Ca2+-dependent fashion. Calmodulin and Mg2+, but not heparin, modulated channel activity in the presence of cADP-ribose. We propose that these Ca2+ channels constitute the intracellular Ca2+-induced Ca2+ release pathway that is activated by cADP-ribose in sea urchin eggs.

calcium release channels; ryanodine receptors; intracellular calcium; oocytes; endoplasmic reticulum; calmodulin; intracellular channels


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
D. J. Turner, B. J. Segura, R. A. Cowles, W. Zhang, and M. W. Mulholland
Functional overlap of IP3- and cADP-ribose-sensitive calcium stores in guinea pig myenteric neurons
Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G208 - G215.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Shiwa, T. Murayama, and Y. Ogawa
Molecular cloning and characterization of ryanodine receptor from unfertilized sea urchin eggs
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2002; 282(3): R727 - R737.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online