| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts 02118
The glycolytic enzyme glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) is induced by hypoxia in endothelial cells (EC).
To define the mechanisms by which GAPDH is regulated by hypoxia, EC
were exposed to cobalt, other transition metals, carbon monoxide (CO),
deferoxamine, or cycloheximide in the presence or absence of hypoxia for 24 h, and GAPDH protein and mRNA levels were measured. GAPDH was induced in cells by the transition metals cobalt, nickel, and
manganese and by deferoxamine, and GAPDH mRNA induction by hypoxia was
blocked by cycloheximide. GAPDH induction by hypoxia, unlike that of
other hypoxia-regulated genes, was not inhibited by CO or by
4,6-dioxoheptanoic acid, an inhibitor of heme synthesis. GAPDH
induction was not altered by mediators of protein phosphorylation, a
calcium channel blocker, a calcium ionophore, or alterations in redox
state. GAPDH induction by hypoxia or transitional metals was partially
blocked by sodium nitroprusside but was not altered by the inhibitor of
nitric oxide synthase
N 
-nitro-L-arginine. These
findings suggest that GAPDH induction by hypoxia in EC occurs via
mechanisms other than those involved in other hypoxia-responsive
systems.
hypoxia; endothelium; erythropoietin; nitric oxide; cobalt
This article has been cited by other articles:
|
|
 |
|
  D. G. Peters, W. Ning, T. J. Chu, C. J. Li, and A. M. K. Choi Comparative SAGE analysis of the response to hypoxia in human pulmonary and aortic endothelial cells Physiol Genomics, September 14, 2006; 26(2): 99 - 108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Ning, T. J. Chu, C. J. Li, A. M. K. Choi, and D. G. Peters Genome-wide analysis of the endothelial transcriptome under short-term chronic hypoxia Physiol Genomics, June 17, 2004; 18(1): 70 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Li, S. Oparil, J.-Z. Sun, J. A. Thompson, and Y.-F. Chen Fibroblast growth factor mediates hypoxia-induced endothelin-A receptor expression in lung artery smooth muscle cells J Appl Physiol, August 1, 2003; 95(2): 643 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Zoeller, T. J. Grazia, P. LaCamera, J. Park, D. P. Gaposchkin, and H. W. Farber Increasing plasmalogen levels protects human endothelial cells during hypoxia Am J Physiol Heart Circ Physiol, August 1, 2002; 283(2): H671 - H679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Z. Sun, S. Oparil, P. Lucchesi, J. A. Thompson, and Y.-F. Chen Tyrosine kinase receptor activation inhibits NPR-C in lung arterial smooth muscle cells Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L155 - L163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Scarfo, P. F. Weller, and H. W. Farber Induction of endothelial cell cytoplasmic lipid bodies during hypoxia Am J Physiol Heart Circ Physiol, January 1, 2001; 280(1): H294 - H301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wodopia, H. S. Ko, J. Billian, R. Wiesner, P. Bartsch, and H. Mairbaurl Hypoxia decreases proteins involved in epithelial electrolyte transport in A549 cells and rat lung Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1110 - L1119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. McDowell, K. Gammon, C. J. Bachurski, J. S. Wiest, J. E. Leikauf, D. R. Prows, and G. D. Leikauf Differential Gene Expression in the Initiation and Progression of Nickel-Induced Acute Lung Injury Am. J. Respir. Cell Mol. Biol., October 1, 2000; 23(4): 466 - 474. [Abstract] [Full Text]
|
|
|
|
 |
|
 O. Meilhac, M. Zhou, N. Santanam, and S. Parthasarathy Lipid peroxides induce expression of catalase in cultured vascular cells J. Lipid Res., August 1, 2000; 41(8): 1205 - 1213. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. E. Godfrey, S.-H. Kim, M. Chavira, D. W. Ruff, R. S. Warren, J. W. Gray, and R. H. Jensen Quantitative mRNA Expression Analysis from Formalin-Fixed, Paraffin-Embedded Tissues Using 5' Nuclease Quantitative Reverse Transcription-Polymerase Chain Reaction J. Mol. Diagn., May 1, 2000; 2(2): 84 - 91. [Abstract] [Full Text]
|
|
|
|
|
|
S. R. Riddle, A. Ahmad, S. Ahmad, S. S. Deeb, M. Malkki, B. K. Schneider, C. B. Allen, and C. W. White Hypoxia induces hexokinase II gene expression in human lung cell line A549 Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L407 - L416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Denko, C. Schindler, A. Koong, K. Laderoute, C. Green, and A. Giaccia Epigenetic Regulation of Gene Expression in Cervical Cancer Cells by the Tumor Microenvironment Clin. Cancer Res., February 1, 2000; 6(2): 480 - 487. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. V. Panchenko, H. W. Farber, and J. H. Korn Induction of heme oxygenase-1 by hypoxia and free radicals in human dermal fibroblasts Am J Physiol Cell Physiol, January 1, 2000; 278(1): C92 - C101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Ebert and H. F. Bunn Regulation of the Erythropoietin Gene Blood, September 15, 1999; 94(6): 1864 - 1877. [Full Text] [PDF]
|
|
|
|
 |
|
 V. Srinivas, X. Zhu, S. Salceda, R. Nakamura, and J. Caro Hypoxia-inducible Factor 1alpha (HIF-1alpha ) Is a Non-heme Iron Protein. IMPLICATIONS FOR OXYGEN SENSING J. Biol. Chem., July 17, 1998; 273(29): 18019 - 18022. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
