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Am J Physiol Cell Physiol 274: C333-C340, 1998;
0363-6143/98 $5.00
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Vol. 274, Issue 2, C333-C340, February 1998

Clminus currents activated via purinergic receptors in Xenopus follicles

Rogelio O. Arellano1, Edith Garay1, and Ricardo Miledi1,2

1 Centro de Neurobiología, Universidad Nacional Autónoma de México, Queretaro, Queretaro 76001, Mexico; and 2 Laboratory of Cellular and Molecular Neurobiology, University of California, Irvine, California 92697-4550

Ionic currents elicited via purinergic receptors located in the membrane of Xenopus follicles were studied using electrophysiological techniques. Follicles responded to ATP-activating inward currents with a fast time course (Fin). In Ringer solution, reversal potential (Erev) of Fin was -22 mV, which did not change with external substitutions of Na+ or K+, whereas solutions containing 50 or 5% of normal Cl- concentration shifted Erev to about +4 and +60 mV, respectively, and decreased Fin amplitude, indicating that Fin was carried by Cl-. Fin had an onset delay of ~400 ms, measured by application of a brief jet of ATP from a micropipette positioned near the follicle (50 µm). Fin was inhibited by 50% in follicles pretreated with pertussis toxin. This suggests a G protein-mediated receptor channel pathway. Fin was mimicked by 2-MeSATP and UTP, the potency order (half-maximal effective concentration) was 2-MeSATP (194 nM) > UTP (454 nM) > ATP (1,086 nM). All agonists generated Cl- currents and displayed cross-inhibition on the others. Fin activation by acetylcholine also cross-inhibited Fin-ATP responses, suggesting that all act on a common channel-activation pathway.

chloride channels; Xenopus oocytes; UTP receptors; follicular cells


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