| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-opioid receptor
Department of Physiology and Institute of Cardiovascular Science and Medicine, Faculty of Medicine, The University of Hong Kong, Hong Kong
To determine whether
the phosphoinositol/Ca2+ pathway
interacts with the adenylate cyclase/adenosine 3',5'-cyclic
monophosphate (cAMP) pathway in the cardiac 
-receptor, the effects
of U-50488, a specific -receptor agonist, on the intracellular
Ca2+ concentration
([Ca2+]i)
and forskolin-induced accumulation of cAMP in rat ventricular myocytes
were determined after interference of the
phosphoinositol/Ca2+ pathway.
U-50488 suppressed the forskolin-induced accumulation of cAMP and
elevated
[Ca2+]i,
which were blocked by norbinaltorphimine, a specific -receptor antagonist, and pertussis toxin. The effects of U-50488 were
qualitatively similar to those of A-23187, a
Ca2+ ionophore, but opposite to
those of
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA)-acetoxymethyl ester (AM), a
[Ca2+]i
chelator. Abolition of U-50488-induced elevation of
[Ca2+]i
by BAPTA-AM also abolished the effect of U-50488 on forskolin-induced accumulation of cAMP. Inhibition of the phospholipase C by specific inhibitors, U-73122 and neomycin, abolished the effects of U-50488 on
both
[Ca2+]i
and forskolin-induced accumulation of cAMP. The results showed for the
first time that -receptor stimulation may suppress cAMP accumulation
via activation of the
phosphoinositol/Ca2+ pathway in
the rat heart.
adenylate cyclase; intracellular calcium ion; phospholipase C; ventricular myocyte; adenosine 3',5'-cyclic monophosphate
This article has been cited by other articles:
|
|
 |
|
  D. A. Jacobson, J. Cho, L. R. Landa Jr., N. A. Tamarina, M. W. Roe, J. D. Buxbaum, and L. H. Philipson Downstream regulatory element antagonistic modulator regulates islet prodynorphin expression Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E587 - E595. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-Y. Wang, J. J. Zhou, J. Shan, and T.-M. Wong Protein Kinase C-epsilon Is a Trigger of Delayed Cardioprotection against Myocardial Ischemia of kappa -Opioid Receptor Stimulation in Rat Ventricular Myocytes J. Pharmacol. Exp. Ther., November 1, 2001; 299(2): 603 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Pei, J.-J. Zhou, J.-S. Bian, X.-C. Yu, M.-L. Fung, and T.-M. Wong Impaired [Ca2+]i and pHi responses to kappa -opioid receptor stimulation in the heart of chronically hypoxic rats Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1483 - C1494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Li, J.-S. Bian, Y. W. Kwan, and T. M. Wong Enhanced Responses to 17beta -Estradiol in Rat Hearts Treated with Isoproterenol: Involvement of a Cyclic AMP-Dependent Pathway J. Pharmacol. Exp. Ther., May 1, 2000; 293(2): 592 - 598. [Abstract] [Full Text]
|
|
|
|
|
|
J.-S. Bian, W.-M. Zhang, J.-M. Pei, and T.-M. Wong The Role of Phosphodiesterase in Mediating the Effect of Protein Kinase C on Cyclic AMP Accumulation upon kappa -Opioid Receptor Stimulation in the Rat Heart J. Pharmacol. Exp. Ther., March 1, 2000; 292(3): 1065 - 1070. [Abstract] [Full Text]
|
|
|
|
|
|
J.-M. Pei, X.-C. Yu, J.-S. Bian, and T.-M. Wong Acidosis antagonizes intracellular calcium response to kappa -opioid receptor stimulation in the rat heart Am J Physiol Cell Physiol, September 1, 1999; 277(3): C492 - C500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wu, H. Y. Li, and T. M. Wong Cardioprotection of Preconditioning by Metabolic Inhibition in the Rat Ventricular Myocyte : Involvement of {kappa}-Opioid Receptor Circ. Res., June 25, 1999; 84(12): 1388 - 1395. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
