Am J Physiol Cell Physiol Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 274: C182-C191, 1998;
0363-6143/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jakob, I.
Right arrow Articles by Lindemann, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jakob, I.
Right arrow Articles by Lindemann, B.
Vol. 274, Issue 1, C182-C191, January 1998

MDR1 in taste buds of rat vallate papilla: functional, immunohistochemical, and biochemical evidence

Ingrid Jakob1, Ingeborg A. Hauser2, Frank Thévenod1, and Bernd Lindemann1

1 Department of Physiology, Saar University, D-66421 Homburg; and 2 Department of Internal Medicine IV, University of Erlangen-Nürnberg, D-90471 Nürnberg, Germany

Multidrug resistance P-glycoprotein (MDR1) is a membrane protein of 150-170 kDa that catalyzes the ATP-driven efflux of hydrophobic xenobiotics, including fluorescent dyes, from cells. Expressed in many epithelial tissues and in the endothelia of the blood-brain barrier, the MDR1 protein provides major routes of detoxification. We found that taste cells of the rat vallate papilla (VP; posterior tongue) had only a slow increase in fluorescence due to uptake of the hydrophobic dye calcein acetoxymethyl ester. However, the development of fluorescence was accelerated two- to threefold by substrates and/or inhibitors of MDR1, such as verapamil, tamoxifen, and cyclosporin A, and by addition of the transport-blocking antibody to MDR1, UIC2. Western blots of vallate tissue rich in taste buds with the MDR1-specific monoclonal antibodies C219 and C494 revealed an immunoreactive protein at ~170 kDa. In contrast, the lingual epithelium surrounding the VP showed a much weaker band with these antibodies. Furthermore, using the antibodies C494 and UIC2 with tissue sections, MDR1-like immunoreactivity was found in taste cells. These results show that MDR1 is present and functional in vallate taste cells of the rat. MDR1-related transport may achieve active elimination of xenobiotics from the sensory cells and thereby protect the peripheral taste organs from potentially harmful molecules contained in an animal's food.

fura 2-acetoxymethyl ester; calcein-acetoxymethyl ester; 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein-acetoxymethyl ester; C219; C494; UIC2


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Zubare-Samuelov, M. E. Shaul, I. Peri, A. Aliluiko, O. Tirosh, and M. Naim
Inhibition of signal termination-related kinases by membrane-permeant bitter and sweet tastants: potential role in taste signal termination
Am J Physiol Cell Physiol, August 1, 2005; 289(2): C483 - C492.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Thevenod, J. M. Friedmann, A. D. Katsen, and I. A. Hauser
Up-regulation of Multidrug Resistance P-glycoprotein via Nuclear Factor-kappa B Activation Protects Kidney Proximal Tubule Cells from Cadmium- and Reactive Oxygen Species-induced Apoptosis
J. Biol. Chem., January 21, 2000; 275(3): 1887 - 1896.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. A. DeSimone
Focus on "Rapid entry of bitter and sweet tastants into liposomes and taste cells: implications for signal transduction"
Am J Physiol Cell Physiol, January 1, 2000; 278(1): C13 - C16.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
I. Peri, H. Mamrud-Brains, S. Rodin, V. Krizhanovsky, Y. Shai, S. Nir, and M. Naim
Rapid entry of bitter and sweet tastants into liposomes and taste cells: implications for signal transduction
Am J Physiol Cell Physiol, January 1, 2000; 278(1): C17 - C25.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online