Am J Physiol Cell Physiol Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 274: C175-C181, 1998;
0363-6143/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mihailidou, A. S.
Right arrow Articles by Rasmussen, H. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mihailidou, A. S.
Right arrow Articles by Rasmussen, H. H.
Vol. 274, Issue 1, C175-C181, January 1998

Na+ influx and Na+-K+ pump activation during short-term exposure of cardiac myocytes to aldosterone

Anastasia S. Mihailidou1, Kerrie A. Buhagiar1,2, and Helge H. Rasmussen1,2

Department of Cardiology, 1 Royal North Shore Hospital, and 2 University of Sydney, Sydney, New South Wales 2065, Australia

To examine the effect of aldosterone on sarcolemmal Na+ transport, we measured ouabain-sensitive electrogenic Na+-K+ pump current (Ip) in voltage-clamped ventricular myocytes and intracellular Na+ activity (aiNa) in right ventricular papillary muscles. Aldosterone (10 nM) induced an increase in both Ip and the rate of rise of aiNa during Na+-K+ pump blockade with the fast-acting cardiac steroid dihydroouabain. The aldosterone-induced increase in Ip and rate of rise of aiNa was eliminated by bumetanide, suggesting that aldosterone activates Na+ influx through the Na+-K+-2Cl- cotransporter. To obtain independent support for this, the Na+, K+, and Cl- concentrations in the superfusate and solution of pipettes used to voltage clamp myocytes were set at levels designed to abolish the inward electrochemical driving force for the Na+-K+-2Cl- cotransporter. This eliminated the aldosterone-induced increase in Ip. We conclude that in vitro exposure of cardiac myocytes to aldosterone activates the Na+-K+-2Cl- cotransporter to enhance Na+ influx and stimulate the Na+-K+ pump.

ion transport; intracellular sodium; sodium-potassium-two chloride cotransport; mineralocorticoid receptor; cell membrane


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. Boixel, B. Gavillet, J.-S. Rougier, and H. Abriel
Aldosterone increases voltage-gated sodium current in ventricular myocytes
Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2257 - H2266.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. O. Andersen, T. Skomedal, M. Enger, A. Fidjeland, T. Brattelid, F. O. Levy, and J.-B. Osnes
{alpha}1-AR-mediated activation of NKCC in rat cardiomyocytes involves ERK-dependent phosphorylation of the cotransporter
Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1354 - H1360.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
H. G. Glitsch
Electrophysiology of the Sodium-Potassium-ATPase in Cardiac Cells
Physiol Rev, October 1, 2001; 81(4): 1791 - 1826.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
O. M. Sejersted and G. Sjogaard
Dynamics and Consequences of Potassium Shifts in Skeletal Muscle and Heart During Exercise
Physiol Rev, October 1, 2000; 80(4): 1411 - 1481.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
W. G. OLIVERA, D. E. CICCOLELLA, N. BARQUIN, K. M. RIDGE, D. H. RUTSCHMAN, D. B. YEATES, and J. I. SZNAJDER
Aldosterone Regulates Na,K-ATPase and Increases Lung Edema Clearance in Rats
Am. J. Respir. Crit. Care Med., February 1, 2000; 161(2): 567 - 573.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
A. S. Mihailidou, H. Bundgaard, M. Mardini, P. S. Hansen, K. Kjeldsen, and H. H. Rasmussen
Hyperaldosteronemia in Rabbits Inhibits the Cardiac Sarcolemmal Na+-K+ Pump
Circ. Res., January 7, 2000; 86(1): 37 - 42.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J.-P. Benitah and G. Vassort
Aldosterone Upregulates Ca2+ Current in Adult Rat Cardiomyocytes
Circ. Res., December 3, 1999; 85(12): 1139 - 1145.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online