Am J Physiol Cell Physiol Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 273: C2002-C2009, 1997;
0363-6143/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hubatsch, D. A.
Right arrow Articles by Jasmin, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hubatsch, D. A.
Right arrow Articles by Jasmin, B. J.
Vol. 273, Issue 6, C2002-C2009, December 1997

Mechanical stimulation increases expression of acetylcholinesterase in cultured myotubes

Douglas A. Hubatsch and Bernard J. Jasmin

Department of Physiology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada K1H 8M5

We tested the hypothesis that acetylcholinesterase (AChE) expression in skeletal muscle cells is increased by passive mechanical stimulation. To this end, primary cultures of myotubes were subjected to repeated cycles of stretch-relaxation for 5 min, 30 min, 3 h, and 24 h, using the Flexercell FX-2000 strain unit. Although mechanical stimulation did not affect AChE expression at early time points, it led to a significant increase (42%; P < 0.05) in total AChE activity at 24 h. This increase reflected a general elevation in the activity of all AChE molecular forms as opposed to a preferential increase in a specific form. Tetrodotoxin (TTX) treatment did not prevent the increase in AChE expression, whereas nifedipine partially blocked it. These changes in enzyme expression were accompanied by increases in the levels of AChE mRNA, suggesting the involvement of pretranslational regulatory mechanisms. Together, these results illustrate that, in addition to neural activation and trophic factors, passive mechanical forces modulate expression of AChE in skeletal muscle cells. Because TTX did not prevent the increase in AChE expression, it appears that the effects of mechanical stimulation are independent of electrical activity, which further indicates the use of an alternate signaling pathway.

rat; neuromuscular junction; synapse; stretch; mechanical cell stimulator


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Rauch and P. T. Loughna
Static stretch promotes MEF2A nuclear translocation and expression of neonatal myosin heavy chain in C2C12 myocytes in a calcineurin- and p38-dependent manner
Am J Physiol Cell Physiol, March 1, 2005; 288(3): C593 - C605.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
W. Matar, J. A. Lunde, B. J. Jasmin, and J.-M. Renaud
Denervation enhances the physiological effects of the KATP channel during fatigue in EDL and soleus muscle
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2001; 281(1): R56 - R65.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Boudreau-Lariviere, D. J. Parry, and B. J. Jasmin
Myotubes originating from single fast and slow satellite cells display similar patterns of AChE expression
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2000; 278(1): R140 - R148.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online