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-adrenergic receptor
stimulation in mice
Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115; and Cardiovascular and Pulmonary Research Institute, Allegheny University of the Health Sciences, Pittsburgh, Pennsylvania 15212
Caveolae, flask-shaped invaginations of cell membranes, are
believed to play pivotal roles in transmembrane transportation of
molecules and cellular signaling. Caveolin, a structural component of
caveolae, interacts directly with G proteins and regulates their
function. We investigated the effect of chronic 
-adrenergic receptor
stimulation on the expression of caveolin subtypes in mouse hearts by
immunoblotting and Northern blotting. Caveolin-1 and -3 were abundantly
expressed in the heart and skeletal muscles, but not in the brain.
Continuous (
)-isoproterenol, but not (+)-isoproterenol, infusion
via osmotic minipump (30 µg · g
1 · day1)
for 13 days significantly downregulated both caveolin subtypes in the
heart. The expression of caveolin-1 was reduced by 48 ± 6.1% and
that of caveolin-3 by 28 ± 4.0%
(P < 0.01, n = 8 for each). The subcellular
distribution of caveolin subtypes in ventricular myocardium was not
altered as determined by sucrose gradient fractionation. In contrast,
the expression of both caveolin subtypes in skeletal muscles was not
significantly changed. Our data suggest that the expression of caveolin
subtypes is regulated by -adrenergic receptor stimulation in the
heart.
caveolae; G protein; isoproterenol; ventricular myocardium
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