Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 273: C1828-C1834, 1997;
0363-6143/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oonishi, T.
Right arrow Articles by Uyesaka, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oonishi, T.
Right arrow Articles by Uyesaka, N.
Vol. 273, Issue 6, C1828-C1834, December 1997

Regulation of red blood cell filterability by Ca2+ influx and cAMP-mediated signaling pathways

Tadahiro Oonishi, Kanako Sakashita, and Nobuhiro Uyesaka

Nippon Telegraph and Telephone Corporation Medical Research Institute, Kanto Teishin Hospital, Tokyo 141; and Department of Physiology, Nippon Medical School, Tokyo 113, Japan

To investigate the mechanism of the regulation of human red blood cell deformability, we examined the deformability under mechanical stress. Washed human red blood cells were rapidly injected through a fine needle, and their filterability was measured using a nickel mesh filter. The decrease in filterability showed a V-shaped curve depending on the extracellular Ca2+ concentration; the maximum decrease was achieved at ~50 µM. The decreased filterability was accompanied by no change in cell morphology and cell volume, indicating that the decrease in filterability can be ascribed to alterations of the membrane properties. Ca2+ entry blockers (nifedipine and felodipine) inhibited the impairment of filterability under mechanical stress. Prostaglandins E1 and E2, epinephrine, and pentoxifylline, which are thought to modulate the intracellular adenosine 3',5'-cyclic monophosphate (cAMP) level of red blood cells, improved or worsened the impaired filterability according to their expected actions on the cAMP level of the cells. These results strongly suggest that the membrane properties regulating red blood cell deformability are affected by the signal transduction system, including Ca2+-dependent and cAMP-mediated signaling pathways.

calcium entry blockers, prostaglandins, epinephrine, pentoxifylline; adenosine 3',5'-cyclic monophosphate; calcium ion


This article has been cited by other articles:


Home page
 CLIN APPL THROMB HEMOSTHome page
C. Fatini, L. Mannini, E. Sticchi, E. Cecchi, A. Bruschettini, E. Leprini, P. Pagnini, G. F. Gensini, D. Prisco, and R. Abbate
eNOS Gene Affects Red Cell Deformability: Role of T-786C, G894T, and 4a/4b Polymorphisms
Clinical and Applied Thrombosis/Hemostasis, October 1, 2005; 11(4): 481 - 488.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Bor-Kucukatay, R. B. Wenby, H. J. Meiselman, and O. K. Baskurt
Effects of nitric oxide on red blood cell deformability
Am J Physiol Heart Circ Physiol, May 1, 2003; 284(5): H1577 - H1584.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. C. Hines, Q. Zen, S. N. Burney, D. A. Shea, K. I. Ataga, E. P. Orringer, M. J. Telen, and L. V. Parise
Novel epinephrine and cyclic AMP-mediated activation of BCAM/Lu-dependent sickle (SS) RBC adhesion
Blood, April 15, 2003; 101(8): 3281 - 3287.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. A. Andrews, L. Yang, and P. S. Low
Phorbol ester stimulates a protein kinase C-mediated agatoxin-TK-sensitive calcium permeability pathway in human red blood cells
Blood, October 16, 2002; 100(9): 3392 - 3399.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. S. Shin, M.-R. Chin, J. S. Kim, J.-H. Chung, C.-K. Ryu, S. Y. Jung, and D. K. Kim
Purification and Characterization of a Cytosolic, 42-kDa and Ca2+-dependent Phospholipase A2 from Bovine Red Blood Cells. ITS INVOLVEMENT IN Ca2+-DEPENDENT RELEASE OF ARACHIDONIC ACID FROM MAMMALIAN RED BLOOD CELLS
J. Biol. Chem., May 31, 2002; 277(23): 21086 - 21094.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. Yang, D. A. Andrews, and P. S. Low
Lysophosphatidic acid opens a Ca++ channel in human erythrocytes
Blood, April 1, 2000; 95(7): 2420 - 2425.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online