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Am J Physiol Cell Physiol 273: C1811-C1818, 1997;
0363-6143/97 $5.00
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Vol. 273, Issue 6, C1811-C1818, December 1997

Effect of changes in respiratory blood parameters on equine red blood cell K-Cl cotransporter

P. F. Speake1, C. A. Roberts2, and J. S. Gibson1

1 Department of Veterinary Preclinical Sciences, University of Liverpool, Liverpool L69 3BX; and 2 Centre for Equine Studies, Animal Health Trust, Newmarket, Suffolk CB8 7DW, United Kingdom

K influx into equine red blood cells (RBCs) was measured using 86Rb as a tracer for K under conditions designed to mimic the changes in respiratory blood parameters that occur in vivo during strenuous exercise. The effects on K influx of physiological changes in pH, cell volume, O2 tension (PO2), CO2 tension (PCO2), and bicarbonate and lactate concentrations were defined. Physiological PO2 exerted a dominant controlling influence on the H+-stimulated Cl-dependent K influx, consistent with effects on the K-Cl cotransporter; PO2 required for half-maximal activity was 37 ± 3 mmHg (4.9 kPa). Although RBCs were swollen at low pH, results showed explicitly that the volume change per se had little effect on K influx. Lactate had no effect on volume- or H+-stimulated K influxes, nor did bicarbonate or PCO2 affect the magnitude of K influxes after these stimuli or after treatment with protein kinase/phosphatase inhibitors. These results represent the first detailed report of O2 dependence of H+-stimulated K-Cl cotransport in RBCs from any mammalian species. They emphasize the importance of PO2 in control of RBC K-Cl cotransport.

oxygen; pH; bicarbonate; lactate


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