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Vol. 273, Issue 5, C1775-C1782, November 1997
Department of Physiology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois 60153
The subcellular spatial and temporal organization of
agonist-induced Ca2+ signals was
investigated in single cultured vascular endothelial cells.
Extracellular application of ATP initiated a rapid increase of
intracellular Ca2+ concentration
([Ca2+]i)
in peripheral cytoplasmic processes from where activation propagated as
a
[Ca2+]i
wave toward the central regions of the cell. The average propagation velocity of the
[Ca2+]i
wave in the peripheral processes was 20-60 µm/s, whereas in the
central region the wave propagated at <10 µm/s. The time course of
the recovery of
[Ca2+]i
depended on the cell geometry. In the peripheral processes (i.e.,
regions with a high surface-to-volume ratio)
[Ca2+]i
declined monotonically, whereas in the central region
[Ca2+]i
decreased in an oscillatory fashion. Propagating
[Ca2+]i
waves were preceded by small, highly localized
[Ca2+]i
transients originating from 1- to 3-µm-wide regions. The average amplitude of these elementary events of
Ca2+ release was 23 nM, and the
underlying flux of Ca2+ amounted
to ~1-2 × 10
18
mol/s or ~0.3 pA, consistent with a
Ca2+ flux through a single or
small number of endoplasmic reticulum Ca2+-release channels.
confocal microscopy; fluo 3; endoplasmic reticulum; inositol trisphosphate
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