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Vol. 273, Issue 4, C1354-C1361, October 1997
channels of
nonpigmented ciliary epithelial cells
Departments of 1 Physiology and 3 Medicine, The University of Pennsylvania, Philadelphia, Pennsylvania 19104-6085; and 2 Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, Connecticut 06510
Ciliary
epithelial cells possess multiple purinergic receptors, and occupancy
of A1 and
A2 adenosine receptors is
associated with opposing effects on intraocular pressure. Aqueous
adenosine produced increases in short-circuit current across rabbit
ciliary epithelium, blocked by removing
Cl
and enhanced by aqueous
Ba2+. Adenosine's actions were
further studied with nonpigmented ciliary epithelial (NPE) cells from
continuous human HCE and ODM lines and freshly dissected bovine cells.
With gramicidin present, adenosine (
3 µM) triggered isosmotic
shrinkage of the human NPE cells, which was inhibited by the
Cl channel blockers
5-nitro-2-(3-phenylpropylamino)benzoate (NPPB) and niflumic acid. At 10 µM, the nonmetabolizable analog 2-chloroadenosine and AMP also
produced shrinkage, but not inosine, UTP, or ATP. 2-Chloroadenosine
(1 µM) triggered increases of whole cell currents in HCE cells,
which were partially reversible,
Cl dependent, and
reversibly inhibited by NPPB. Adenosine (10 µM) also stimulated
whole cell currents in bovine NPE cells. We conclude that occupancy of
adenosine receptors stimulates
Cl secretion in mammalian
NPE cells.
cell volume; whole cell recording; short-circuit current; 2-chloroadenosine; adenosine 5'-triphosphate; purines
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