Am J Physiol Cell Physiol Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 273: C1290-C1297, 1997;
0363-6143/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ullrich, N.
Right arrow Articles by Sontheimer, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ullrich, N.
Right arrow Articles by Sontheimer, H.

Vol. 273, Issue 4, C1290-C1297, October 1997

Cell cycle-dependent expression of a glioma-specific chloride current: proposed link to cytoskeletal changes

Nicole Ullrich and Harald Sontheimer

Department of Neurobiology, University of Alabama at Birmingham, Birmingham, Alabama 35294

We recently demonstrated expression of a novel, glioma-specific Cl- current in glial-derived tumor cells (gliomas), including stable cell lines such as STTG1, derived from a human anaplastic astrocytoma. We used STTG1 cells to study whether glioma Cl- channel (GCC) activity is regulated during cell cycle progression. Cells were arrested in defined stages of cell cycle (G0, G1, G1/S, S, and M phases) using serum starvation, mevastatin, hydroxyurea, demecolcine, and cytosine beta -D-arabinofuranoside. Cell cycle arrest was confirmed by measuring [3H]thymidine incorporation and by DNA flow cytometry. Using whole cell patch-clamp recordings, we demonstrate differential changes in GCC activity after cell proliferation and cell cycle progression was selectively altered; specifically, channel expression was low in serum-starved, G0-arrested cells, increased significantly in early G1, decreased during S phase, and increased after arrest in M phase. Although the link between the cell cycle and GCC activity is not yet clear, we speculate that GCCs are linked to the cytoskeleton and that cytoskeletal rearrangements associated with cell division lead to the observed changes in channel activity. Consistent with this hypothesis, we demonstrate the activation of GCC by disruption of F-actin using cytochalasin D or osmotic cell swelling.

ion channels; flow cytometry; proliferation; chlorotoxin; glioblastoma; astrocytoma


This article has been cited by other articles:


Home page
 HypertensionHome page
W. Liang, J. B. Ray, J. Z. He, P. H. Backx, and M. E. Ward
Regulation of Proliferation and Membrane Potential by Chloride Currents in Rat Pulmonary Artery Smooth Muscle Cells
Hypertension, August 1, 2009; 54(2): 286 - 293.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. E. Loewen and G. W. Forsyth
Structure and Function of CLCA Proteins
Physiol Rev, July 1, 2005; 85(3): 1061 - 1092.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Chen, L. Wang, L. Zhu, S. Nie, J. Zhang, P. Zhong, B. Cai, H. Luo, and T. J. C. Jacob
Cell cycle-dependent expression of volume-activated chloride currents in nasopharyngeal carcinoma cells
Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1313 - C1323.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Kolajova, M.-A. Hammer, J. L. Collins, and J. M. Baltz
Developmentally regulated cell cycle dependence of swelling-activated anion channel activity in the mouse embryo
Development, September 15, 2001; 128(18): 3427 - 3434.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S. M. Duffy, M. L. Leyland, E. C. Conley, and P. Bradding
Voltage-dependent and calcium-activated ion channels in the human mast cell line HMC-1
J. Leukoc. Biol., August 1, 2001; 70(2): 233 - 240.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. J. Copeland, B. K. Berdiev, H.-L. Ji, J. Lockhart, S. Parker, C. M. Fuller, and D. J. Benos
Regions in the carboxy terminus of {alpha}-bENaC involved in gating and functional effects of actin
Am J Physiol Cell Physiol, July 1, 2001; 281(1): C231 - C240.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Czarnecki, S. Vaur, L. Dufy-Barbe, B. Dufy, and L. Bresson-Bepoldin
Cell cycle-related changes in transient K+ current density in the GH3 pituitary cell line
Am J Physiol Cell Physiol, December 1, 2000; 279(6): C1819 - C1828.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. Soroceanu, T. J. Manning Jr, and H. Sontheimer
Modulation of Glioma Cell Migration and Invasion Using Cl- and K+ Ion Channel Blockers
J. Neurosci., July 15, 1999; 19(14): 5942 - 5954.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Eder, R. Klee, and U. Heinemann
Involvement of Stretch-Activated Cl- Channels in Ramification of Murine Microglia
J. Neurosci., September 15, 1998; 18(18): 7127 - 7137.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C. Elble and B. U. Pauli
Tumor Suppression by a Proapoptotic Calcium-activated Chloride Channel in Mammary Epithelium
J. Biol. Chem., October 26, 2001; 276(44): 40510 - 40517.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online