Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 273: C1250-C1258, 1997;
0363-6143/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grover, A. K.
Right arrow Articles by Samson, S. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grover, A. K.
Right arrow Articles by Samson, S. E.

Vol. 273, Issue 4, C1250-C1258, October 1997

Peroxide resistance of ER Ca2+ pump in endothelium: implications to coronary artery function

Ashok K. Grover and Sue E. Samson

Department of Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada L8N 3Z5

We examined the effects of peroxide on the sarco(endo)plasmic reticulum Ca2+ (SERCA) pump in pig coronary artery endothelium and smooth muscle at three organizational levels: Ca2+ transport in permeabilized cells, cytosolic Ca2+ concentration in intact cells, and contractile function of artery rings. We monitored the ATP-dependent, azide-insensitive, oxalate-stimulated 45Ca2+ uptake by saponin-permeabilized cultured cells. Low concentrations of peroxide inhibited the uptake less effectively in endothelium than in smooth muscle whether we added the peroxide directly to the Ca2+ uptake solution or treated intact cells with peroxide and washed them before the permeabilization. An acylphosphate formation assay confirmed the greater resistance of the SERCA pump in endothelial cells than in smooth muscle cells. Pretreating smooth muscle cells with 300 µM peroxide inhibited (by 77 ± 2%) the cyclopiazonic acid (CPA)-induced increase in cytosolic Ca2+ concentration in a Ca2+-free solution, but it did not affect the endothelial cells. Peroxide pretreatment inhibited the CPA-induced contraction in deendothelialized arteries with a 50% inhibitory concentration of 97 ± 13 µM, but up to 500 µM peroxide did not affect the endothelium-dependent, CPA-induced relaxation. Similarly, 500 µM peroxide inhibited the angiotensin-induced contractions in deendothelialized arteries by 93 ± 2%, but it inhibited the bradykinin-induced, endothelium-dependent relaxation by only 40 ± 13%. The greater resistance of the endothelium to reactive oxygen may be important during ischemia-reperfusion or in the postinfection immune response.

free radicals; adenosinetriphosphatase; bradykinin; angiotensin; cyclopiazonic acid; thapsigargin; fluorescence; ischemia ; endoplasmic reticulum


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. J. Kennedy, S. Vetteth, M. Xie, S. M. Periyasamy, Z. Xie, C. Han, V. Basrur, K. Mutgi, V. Fedorov, D. Malhotra, et al.
Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation
Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H3003 - H3011.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Pande, K. K. Mallhi, A. Sawh, M. M. Szewczyk, F. Simpson, and A. K. Grover
Aortic smooth muscle and endothelial plasma membrane Ca2+ pump isoforms are inhibited differently by the extracellular inhibitor caloxin 1b1
Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1341 - C1349.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. M. Brown, K. Donaldson, P. J. Borm, R. P. Schins, M. Dehnhardt, P. Gilmour, L. A. Jimenez, and V. Stone
Calcium and ROS-mediated activation of transcription factors and TNF-{alpha} cytokine gene expression in macrophages exposed to ultrafine particles
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L344 - L353.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. K. Grover, C.-Y. Kwan, and S. E. Samson
Effects of peroxynitrite on sarco/endoplasmic reticulum Ca2+ pump isoforms SERCA2b and SERCA3a
Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1537 - C1543.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Walia, S. E. Samson, T. Schmidt, K. Best, M. Whittington, C. Y. Kwan, and A. K. Grover
Peroxynitrite and nitric oxide differ in their effects on pig coronary artery smooth muscle
Am J Physiol Cell Physiol, March 1, 2003; 284(3): C649 - C657.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. K. Grover, S. E. Samson, S. Robinson, and C. Y. Kwan
Effects of peroxynitrite on sarcoplasmic reticulum Ca2+ pump in pig coronary artery smooth muscle
Am J Physiol Cell Physiol, February 1, 2003; 284(2): C294 - C301.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. J. Janssen, S. J. Netherton, and D. K. Walters
Ca2+-dependent K+ channels and Na+-K+-ATPase mediate H2O2- and superoxide-induced relaxations in canine trachealis
J Appl Physiol, February 1, 2000; 88(2): 745 - 752.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
Q. Hu, Y. Xia, S. Corda, J. L. Zweier, and R. C. Ziegelstein
Hydrogen Peroxide Decreases pHi in Human Aortic Endothelial Cells by Inhibiting Na+/H+ Exchange
Circ. Res., September 21, 1998; 83(6): 644 - 651.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. M. Moore, P. M. Chetham, J. J. Kelly, and T. Stevens
Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP
Am J Physiol Lung Cell Mol Physiol, August 1, 1998; 275(2): L203 - L222.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online