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Am J Physiol Cell Physiol 273: C1168-C1175, 1997;
0363-6143/97 $5.00
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Vol. 273, Issue 4, C1168-C1175, October 1997

Ca2+-sensing receptors in intestinal epithelium

Lucio Gama1, Lynn M. Baxendale-Cox2, and Gerda E. Breitwieser1

1 Department of Physiology, School of Medicine and 2 School of Nursing, Johns Hopkins University, Baltimore, Maryland 21205

Expression of Ca2+-sensing receptors (CaR) was demonstrated in several human intestinal epithelial cell lines (T84, HT-29, and Caco-2) and in rat intestinal epithelium by both reverse transcriptase-polymerase chain reaction (PCR) and Northern blotting of RNA. Restriction patterns of the PCR products were of the sizes predicted by the human and rat sequences. CaR agonists (Ca2+, poly-L-arginine, protamine) mediated an increase in intracellular Ca2+ in HT-29-18-C1 cells (monitored by changes in fura 2 fluorescence), which was dependent on release from thapsigargin-sensitive stores. U-73122, an inhibitor of phosphatidylinositol-phospholipase C, eliminated the CaR agonist-mediated rise in intracellular Ca2+, whereas its inactive analog, U-73343, had no effect. Pertussis toxin pretreatment had no effect on CaR agonist-mediated modulation of intracellular Ca2+. Taken together, these studies demonstrate that CaR are expressed in intestinal epithelial cells and couple to mobilization of intracellular Ca2+. The presence of CaR in intestinal epithelial cells presents a new locus for investigations into the role(s) of extracellular Ca2+ in modulating intestinal epithelial cell differentiation and transepithelial Ca2+ transport.

calcium-sensing receptor; polycation receptor; G protein-coupled receptors; intracellular calcium


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