Am J Physiol Cell Physiol Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 273: C874-C882, 1997;
0363-6143/97 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duranton, C.
Right arrow Articles by Poujeol, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duranton, C.
Right arrow Articles by Poujeol, P.

AJP - Cell Physiology, Vol 273, Issue 3 C874-C882, Copyright © 1997 by American Physiological Society

ARTICLES

Chloride channels in primary cultures of seawater fish (Dicentrarchus labrax) gill

C. Duranton, M. Tauc, M. Avella and P. Poujeol
Unite Mixte de Recherche Centre National de la Recherche Scientifique 6548, Universite de Nice-Sophia Antipolis, France.

Patch-clamp experiments were undertaken on primary cultures of respiratory cells originating from sea bass (Dicentrarchus labrax) gills. A small-conductance Cl- channel of 8 pS was characterized in cell-attached configuration with 140 mM N-methyl-D-glucamine-Cl in the pipette and bath solutions. No activity was recorded below a membrane holding potential of +20 mV (-Vp, referenced to the pipette solution), and the channel showed an inward rectification. In the inside-out configuration the Cl- channel was active at all membrane holding potentials. Its open probability strongly increased with membrane depolarization. The channel activity could be increased by the application of protein kinase A+ATP. This channel was inhibited by 5-nitro-2-(3-phenylpropylamino)-benzoic acid, diphenylamino-2-carboxylic acid, and I- and was insensitive to 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. The presence of Cl- channels in the apical membrane of respiratory cells provides additional evidence for an important role of this cell type in the control of ion homeostasis of seawater fish.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Duranton, E. Mikulovic, M. Tauc, M. Avella, and P. Poujeol
Potassium channels in primary cultures of seawater fish gill cells. I. Stretch-activated K+ channels
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2000; 279(5): R1647 - R1658.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Duranton, E. Mikulovic, M. Tauc, M. Avella, and P. Poujeol
Potassium channels in primary cultures of seawater fish gill cells. II. Channel activation by hypotonic shock
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2000; 279(5): R1659 - R1670.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online