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Am J Physiol Cell Physiol 273: C790-C803, 1997;
0363-6143/97 $5.00
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AJP - Cell Physiology, Vol 273, Issue 3 C790-C803, Copyright © 1997 by American Physiological Society

ARTICLES

Isolated bovine retinal pigment epithelial cells express delayed rectifier type and M-type K+ currents

M. Takahira and B. A. Hughes
Department of Ophthalmology, University of Michigan, Ann Arbor 48105, USA.

Outwardly rectifying K+ currents in freshly isolated bovine retinal pigment epithelial (RPE) cells were characterized using the whole cell and perforated-patch configurations of the patch-clamp technique. All cells exhibited a delayed rectifier type K+ current. This current had an activation threshold voltage of approximately -40 mV, activated with a sigmoidal trajectory, and inactivated completely over a period of several seconds. External tetraethylammonium (TEA) was an effective blocker of the delayed rectifier current [apparent dissociation constant (Kd) = 5.1 mM], but external Ba2+ was relatively ineffective. Approximately 24% of the cells also exhibited a sustained outwardly rectifying K+ current that became activated at voltages positive to approximately -80 mV. This current resembled the neuronal M-current. External Ba2+ was a potent blocker of this current (apparent Kd = 1.1 mM), but external TEA and Cs+ were relatively ineffective. These results indicate that freshly isolated bovine RPE cells express K+ currents of both the delayed rectifier and M types. The latter may contribute to the resting K+ conductances of the apical and basolateral membranes.


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