AJP - Cell Physiology, Vol 273, Issue 3 C1082-C1087, Copyright © 1997 by American Physiological Society

ARTICLES

Effects of epinephrine on insulin-stimulated glucose uptake and GLUT-4 phosphorylation in muscle

A. D. Lee, P. A. Hansen, J. Schluter, E. A. Gulve, J. Gao and J. O. Holloszy
Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

beta-Adrenergic stimulation has been reported to inhibit insulin-stimulated glucose transport in adipocytes. This effect has been attributed to a decrease in the intrinsic activity of the GLUT-4 isoform of the glucose transporter that is mediated by phosphorylation of GLUT-4. Early studies showed no inhibition of insulin-stimulated glucose transport by epinephrine in skeletal muscle. The purpose of this study was to determine the effect of epinephrine on GLUT-4 phosphorylation, and reevaluate the effect of beta-adrenergic stimulation on insulin-activated glucose transport, in skeletal muscle. We found that 1 microM epinephrine, which raised adenosine 3',5'-cyclic monophosphate approximately ninefold, resulted in GLUT-4 phosphorylation in rat skeletal muscle but had no inhibitory effect on insulin-stimulated 3-O-methyl-D-glucose (3-MG) transport. In contrast to 3-MG transport, the uptakes of 2-deoxyglucose and glucose were markedly inhibited by epinephrine treatment. This inhibitory effect was presumably mediated by stimulation of glycogenolysis, which resulted in an increase in glucose 6-phosphate concentration to levels known to severely inhibit hexokinase. We conclude that 1) beta-adrenergic stimulation decreases glucose uptake by raising glucose 6-phosphate concentration, thus inhibiting hexokinase, but does not inhibit insulin-stimulated glucose transport and 2) phosphorylation of GLUT-4 has no effect on glucose transport in skeletal muscle.

This article has been cited by other articles:

				D. S. Battram, T. E. Graham, and F. Dela Caffeine's impairment of insulin-mediated glucose disposal cannot be solely attributed to adrenaline in humans J. Physiol., September 15, 2007; 583(3): 1069 - 1077. [Abstract] [Full Text] [PDF]

				D. S. Battram, J. Bugaresti, J. Gusba, and T. E. Graham Acute caffeine ingestion does not impair glucose tolerance in persons with tetraplegia J Appl Physiol, January 1, 2007; 102(1): 374 - 381. [Abstract] [Full Text] [PDF]

				A. H. Mulder, C. J. Tack, A. J. Olthaar, P. Smits, F. C. G. J. Sweep, and R. R. Bosch Adrenergic receptor stimulation attenuates insulin-stimulated glucose uptake in 3T3-L1 adipocytes by inhibiting GLUT4 translocation Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E627 - E633. [Abstract] [Full Text] [PDF]

				J. L. Smith, J.-S. Ju, B. M. Saha, B. A. Racette, and J. S. Fisher Levodopa with carbidopa diminishes glycogen concentration, glycogen synthase activity, and insulin-stimulated glucose transport in rat skeletal muscle J Appl Physiol, December 1, 2004; 97(6): 2339 - 2346. [Abstract] [Full Text] [PDF]

				A. R. Gosmanov, G. E. Umpierrez, A. H. Karabell, R. Cuervo, and D. B. Thomason Impaired expression and insulin-stimulated phosphorylation of Akt-2 in muscle of obese patients with atypical diabetes Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E8 - E15. [Abstract] [Full Text] [PDF]

				D. G. Hunt and J. L. Ivy Epinephrine inhibits insulin-stimulated muscle glucose transport J Appl Physiol, November 1, 2002; 93(5): 1638 - 1643. [Abstract] [Full Text] [PDF]

				M. J. Watt and M. Hargreaves Effect of epinephrine on glucose disposal during exercise in humans: role of muscle glycogen Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E578 - E583. [Abstract] [Full Text] [PDF]

				D. G. Hunt, Z. Ding, and J. L. Ivy Propranolol prevents epinephrine from limiting insulin-stimulated muscle glucose uptake during contraction J Appl Physiol, August 1, 2002; 93(2): 697 - 704. [Abstract] [Full Text] [PDF]

				F. S. L. Thong and T. E. Graham Caffeine-induced impairment of glucose tolerance is abolished by beta -adrenergic receptor blockade in humans J Appl Physiol, June 1, 2002; 92(6): 2347 - 2352. [Abstract] [Full Text] [PDF]

				D. G. Hunt, Z. Ding, and J. L. Ivy Clenbuterol prevents epinephrine from antagonizing insulin-stimulated muscle glucose uptake J Appl Physiol, March 1, 2002; 92(3): 1285 - 1292. [Abstract] [Full Text] [PDF]

				F. S.L. Thong, W. Derave, B. Kiens, T. E. Graham, B. Urso, J. F.P. Wojtaszewski, B. F. Hansen, and E. A. Richter Caffeine-Induced Impairment of Insulin Action but Not Insulin Signaling in Human Skeletal Muscle Is Reduced by Exercise Diabetes, March 1, 2002; 51(3): 583 - 590. [Abstract] [Full Text] [PDF]

				J. S. Greiwe, J. O. Holloszy, and C. F. Semenkovich Exercise induces lipoprotein lipase and GLUT-4 protein in muscle independent of adrenergic-receptor signaling J Appl Physiol, July 1, 2000; 89(1): 176 - 181. [Abstract] [Full Text] [PDF]

				R. J. Geor, K. W. Hinchcliff, L. J. McCutcheon, and R. A. Sams Epinephrine inhibits exogenous glucose utilization in exercising horses J Appl Physiol, May 1, 2000; 88(5): 1777 - 1790. [Abstract] [Full Text] [PDF]

				S. Egert, N. Nguyen, and M. Schwaiger Contribution of {alpha}-Adrenergic and ß-Adrenergic Stimulation to Ischemia-Induced Glucose Transporter (GLUT) 4 and GLUT1 Translocation in the Isolated Perfused Rat Heart Circ. Res., June 25, 1999; 84(12): 1407 - 1415. [Abstract] [Full Text] [PDF]

				R. Aslesen and J. Jensen Effects of epinephrine on glucose metabolism in contracting rat skeletal muscles Am J Physiol Endocrinol Metab, September 1, 1998; 275(3): E448 - E456. [Abstract] [Full Text] [PDF]

				A. L. Castle, C.-H. Kuo, D.-H. Han, and J. L. Ivy Amylin-mediated inhibition of insulin-stimulated glucose transport in skeletal muscle Am J Physiol Endocrinol Metab, September 1, 1998; 275(3): E531 - E536. [Abstract] [Full Text] [PDF]