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Am J Physiol Cell Physiol 273: C548-C557, 1997;
0363-6143/97 $5.00
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AJP - Cell Physiology, Vol 273, Issue 2 C548-C557, Copyright © 1997 by American Physiological Society

ARTICLES

Prostaglandin E2 increases 7-pS Cl- channel density in the apical membrane of A6 distal nephron cells

K. E. Kokko, P. S. Matsumoto, Z. R. Zhang, B. N. Ling and D. C. Eaton
Emory University School of Medicine, Department of Physiology, Atlanta, Georgia, USA.

In A6 distal nephron cells, short-circuit current (Isc) was increased by basolateral exposure to prostaglandin E2 (PGE2; peak response at 1 microM). The effect was only partially abolished by either apical amiloride, an Na+ channel blocker, or 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), a Cl- channel blocker. In apical cell-attached patches, we observed a 7-pS Cl- channel with a linear current-voltage relationship, a reversal potential near resting membrane potential, and open probability > 0.5. The channel was blocked by diphenylamine-2-carboxylate, glibenclamide, and NPPB but not by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. The frequency of observed Cl- channel activity increased 7-fold with 10-min exposure to PGE2 and 3.7-fold with longer (10-50 min) exposure to PGE2. The PGE2-induced increase in Cl- channel activity was due primarily to an increase in the number of functional channels. The following conclusions were made: 1) activation of apical, 7-pS Cl- channels in A6 cells accounts for the PGE2-induced increase in the amiloride-insensitive Isc, and 2) 7-pS Cl- channel activation was mediated via an increase in channel density without substantial effects on channel kinetics.


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