AJP - Cell Physiology, Vol 273, Issue 2 C541-C547, Copyright © 1997 by American Physiological Society

ARTICLES

Contribution of a swelling-activated chloride current to changes in the cardiac action potential

J. I. Vandenberg, G. C. Bett and T. Powell
Department of Biochemistry, University of Cambridge, United Kingdom.

The purpose of this investigation was to determine to what extent the swelling-activated Cl- current (ICl,swell) contributes to swelling-induced changes in the resting membrane potential and action potential duration (APD) in ventricular myocytes. Action potentials were recorded from guinea pig ventricular myocytes using conventional whole cell recording techniques. Cell swelling caused initial lengthening followed by a variable shortening of APD. In 59% of cells this secondary APD shortening had a 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS)-sensitive component, consistent with a contribution from ICl,swell. Furthermore, DIDS partially antagonized the depolarization of the resting membrane potential that occurred during cell swelling. We have modeled the ICl,swell using the Oxsoft Heart computer program. Action potential changes predicted by the model agree well with the observed DIDS-sensitive component of the change in the action potential during cell swelling. We conclude that activation of ICl,swell contributes to shortening of APD and depolarization of the resting membrane potential during cell swelling in cardiac myocytes.

This article has been cited by other articles:

				D. Duan Phenomics of cardiac chloride channels: the systematic study of chloride channel function in the heart J. Physiol., May 15, 2009; 587(10): 2163 - 2177. [Abstract] [Full Text] [PDF]

				Z. Ren, F. J. Raucci Jr., D. M. Browe, and C. M. Baumgarten Regulation of swelling-activated Cl- current by angiotensin II signalling and NADPH oxidase in rabbit ventricle Cardiovasc Res, January 1, 2008; 77(1): 73 - 80. [Abstract] [Full Text] [PDF]

				K Terashima, A Takeuchi, N Sarai, S Matsuoka, E.B Shim, C.H Leem, and A Noma Modelling Cl- homeostasis and volume regulation of the cardiac cell Phil Trans R Soc A, May 15, 2006; 364(1842): 1245 - 1265. [Abstract] [Full Text] [PDF]

				Z. Ren and C. M. Baumgarten Antagonistic regulation of swelling-activated Cl- current in rabbit ventricle by Src and EGFR protein tyrosine kinases Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2628 - H2636. [Abstract] [Full Text] [PDF]

				X.-L. Du, Z. Gao, C.-P. Lau, S.-W. Chiu, H.-F. Tse, C. M. Baumgarten, and G.-R. Li Differential Effects of Tyrosine Kinase Inhibitors on Volume-sensitive Chloride Current in Human Atrial Myocytes: Evidence for Dual Regulation by Src and EGFR Kinases J. Gen. Physiol., March 29, 2004; 123(4): 427 - 439. [Abstract] [Full Text] [PDF]

				I. Kocic, Y. Hirano, and M. Hiraoka Ionic basis for membrane potential changes induced by hypoosmotic stress in guinea-pig ventricular myocytes Cardiovasc Res, July 1, 2001; 51(1): 59 - 70. [Abstract] [Full Text] [PDF]

				F. C. Britton, W. J. Hatton, C. F. Rossow, D. Duan, J. R. Hume, and B. Horowitz Molecular distribution of volume-regulated chloride channels (ClC-2 and ClC-3) in cardiac tissues Am J Physiol Heart Circ Physiol, November 1, 2000; 279(5): H2225 - H2233. [Abstract] [Full Text] [PDF]

				F. Brette, S. C. Calaghan, S. Lappin, E. White, J. Colyer, and J.-Y. Le Guennec Biphasic effects of hyposmotic challenge on excitation-contraction coupling in rat ventricular myocytes Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1963 - H1971. [Abstract] [Full Text] [PDF]

				D. Duan, L. Ye, F. Britton, B. Horowitz, and J. R. Hume A Novel Anionic Inward Rectifier in Native Cardiac Myocytes Circ. Res., March 3, 2000; 86 (4): e63 - e71. [Abstract] [Full Text] [PDF]

				J. R. Hume, D. Duan, M. L. Collier, J. Yamazaki, and B. Horowitz Anion Transport in Heart Physiol Rev, January 1, 2000; 80(1): 31 - 81. [Abstract] [Full Text] [PDF]

				K. R. Wong, A. E. O. Trezise, S. Bryant, G. Hart, and J. I. Vandenberg Molecular and functional distributions of chloride conductances in rabbit ventricle Am J Physiol Heart Circ Physiol, October 1, 1999; 277(4): H1403 - H1409. [Abstract] [Full Text] [PDF]

				E. Carmeliet Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias Physiol Rev, July 1, 1999; 79(3): 917 - 1017. [Abstract] [Full Text] [PDF]

				H. F. Clemo, B. S. Stambler, and C. M. Baumgarten Swelling-Activated Chloride Current Is Persistently Activated in Ventricular Myocytes From Dogs With Tachycardia-Induced Congestive Heart Failure Circ. Res., February 5, 1999; 84(2): 157 - 165. [Abstract] [Full Text] [PDF]

				G. M. Dick, K. K. Bradley, B. Horowitz, J. R. Hume, and K. M. Sanders Functional and molecular identification of a novel chloride conductance in canine colonic smooth muscle Am J Physiol Cell Physiol, October 1, 1998; 275(4): C940 - C950. [Abstract] [Full Text] [PDF]